1998 Fiscal Year Final Research Report Summary
Bidirectional transport by ciliated epithelia : mechanism and implications
| Project/Area Number |
09640814
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
動物生理・代謝
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| Research Institution | International Christian University |
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Principal Investigator |
TAKAHASHI Keiichi International Christian University Division of Natural Sciences, Professor, 理学研究科, 教授 (40011481)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Ciliated epithelia / echinoderm / counter-current mechanism |
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| Research Abstract |
The aim of this study was to investigate the possibility that bidirectiona1 transport that had been observed in the madreporite of echinoderms is an inherent property of ciliated epithelia in general. It also aimed to clarify the biological implications of this phenomenon , especially from the viewpoint of possible counter-current exchange mechanism. The transport of particles and water was studied mainly in isolated pore tubes of the sea urchin madreporite using colloidal carbon, polystyrene microbeads, and sulforhodamine 101, a water-soluble fluorescent dye. Observations were made under an inverted differential interference contrast and fluorescence microscope recording with a CCD video camera. The results showed that water is transported predominantly in the direction of the recovery stroke of the cilia while particulate matter was transported in the direction of the effective stroke. Thus, there seems to be a selective mechanism probably based on the size of the particle being transported. Basic data were obtained on the distribution of flow rate in the microscopic domains surrounding the cilia within the pore canals of the madreporite. Preliminary experiments were also carried out using other ciliated epithelia for similar analyses.
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[Publications] Braucker, R., Murakami, A., Ikegaya, K., Yoshimura, K., Takahashi, K., Machemer-Rohnisch, S., and Machemer, H.: "Relaxation and activation of graviresponses in Paramecium caudatum" J.Exp.Biol.201(14). 2103-2113 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Omoto, C.K., Gibbons, I.R., Kamiya, R., Shingyoji, C., Takahashi, K.and Witman, G.B.: "Rotation of the central pair microtubules in eukaryotic flagella" Mol.Biol.Cell.10(1). 1-4 (1999)
Description
「研究成果報告書概要(欧文)」より
