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1998 Fiscal Year Final Research Report Summary

Preparation of a model system for biological solutions and its industrial applications

Research Project

Project/Area Number 09650995
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 高分子構造・物性(含繊維)
Research InstitutionNagoya Institute of Technology

Principal Investigator

KINOSHITA Takatoshi  Nagoya Institute of Technology, Faculty of Engineering, Associate Professor, 工学部, 助教授 (60135407)

Co-Investigator(Kenkyū-buntansha) YOSHIMIZU Hiroaki  Nagoya Institute of Technology, Faculty of Engineering, Assistant Professor, 工学部, 助手 (10240350)
TSUJITA Yoshiharu  Nagoya Institute of Technology, Faculty of Engineering, Professor, 工学部, 教授 (70016591)
Project Period (FY) 1997 – 1998
Keywordsamphiphilic / polypeptides / poly (ethylene glycol) / micelles / solubilization behaviors / orderly packed micelles / plane solutes / model system for biological solutions
Research Abstract

Amphiphilic polypeptides, poly( gamma-methyl L-glutamate)-block-poly(ethylene glycol), PMLG-PEG, and poly(gamma -benzyl L-glutamate)-block-poly(ethylene glycol), PBLG-PEG, were prepared and their aggregation structure and solubilization behaviors were investigated. These amphiphilic polypeptides were soluble in water to form spherical aggregates in which the hydrophobic alpha -helical peptide tail was orderly packed in the hexagonal manner. The solubilization of a plane solute, 1-naphthol, into these aggregates was characterized by semi- equilibrium dialysis (SED) method. Values of the solubilization equilibrium constant(K) decrease as the mole fraction of 1-naphthol in the aggregate (X) increases, And the solubilization isotherm of PBLG-PEG-1-naphthol system is higher as compared with that of PMLG-PEG-1-naphthol system. That is, the aromatic-aromatic interaction in the former system may enhance the solubilization affinity between the aggregate and solute. It is shown, therefore, that the interaction between peptide side chains and solutes is the effective factor to control the solubilization behavior of micellar aggregates composed of peptide-based amphiphiles. Thus, we can confirm the selective solubilization behaviors by the peptide- based orderly packed micelles in aqueous solutions, which may be a simple model system of our blood solutions containing albumin for their anomalous solubilization behaviors.

  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] M.Higuchi 他1名: "“Photocontrol of Molecular Orientation of a Photo-responsive Amphiphilic α-helix in a Lipid Momolayer"" Langmuir. 13.6. 1616-1622 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Toyotama 他4名: "“Controllable Orientation of the Peptide-based Surfactant at Air-Water Interface"" Chem.Lett.1997・5. 443-444 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木下 隆利: "“両親媒性ポリペプチドと分子膜システム"" 高分子. 46・5. 335 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kinoshita 他3名: "“The Molecular Orientation of a Peptide-based Amphiphile at Hexane/Water Interface"" Chem.Lett.1997・8. 745-746 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 広田安信 他4名: "“α-ヘリックス鎖に沿ったクラウンエーテルの一次元的配列制御とその機能"" 高分子論文集. 54・12. 966-973 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kinoshita: "“Photoresponsive Membrane Systems"" Photochem.Photobiol., B : Biology. 42・1. 12-19 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Higuchi 他1名: "“Photoresponsive behavior of self-assembling systems be amphiphilic α-helix with azobenzene unit"" Photochem.Photobiol., B : Biology. 42・1. 143-150 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Kishihara 他3名: "“Vertical standing of Amphiphilic helical peptides at the hexane-water interface"" Chem.Lett.1998・9. 951-952 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Ihara 他6名: "“Detection of molecular-shape recognition for polycyclic aromatic hydrocarbons by α-helical poly(L-alanine) on silica"" Chem.Lett.1998・10. 963-964 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kinoshita 他4名: "“The guest-induced oscillation of a monolayer composed of polypeptide containing β-cycldextrin at the terminal"" Chaos. 9 (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉山圭 他5名: "“ペプチド系ミセルの構造と可溶化挙動"" 高分子論文集. 55 (印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木下 隆利 他16名: "生体工学" 槙書店, 246 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Higuchi, N.Minoura and T.Kinoshita: ""Photocontrol of Molecular Orientation of a Photo-responsive Amphiphilic alpha-helix in a Lipid Momolayer"" Langmuir. Vol.13, No.6. 1616-1622 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Toyotama, s.Kugimiya, M.Yonese, T.Kinoshita and Y.Tsujita: ""Controllable Orientation of the Peptide-based Surfactant at Air-Water Interface"" Chem.Lett.No.5. 443-444 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kinoshita: ""Amphiphilic Polypeptides and Their Molecular Membrane Systems"" Koubunshi. Vol.46, No.5. 335 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Hosokawa, T.Kinoshita, Y.Tsujita and H.Yoshimizu: ""The Molecular Orientation of a Peptide-based pp.Amphiphile at Hexane/Water Interface"" Chem.Lett.No.8. 745-746 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Hirota, F.Nakanishi, T.Kinoshita, Y.Tsujita and H.Yoshimizu: Koubunshi Ronbun-shu. Vol.54, No.12. 966-973 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kinoshita: ""Photoresponsive Membrane Systems"" Photochem.Photobiol., B : Biology. Vol.42, No.1. 12-19 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Higuchi and T.Kinoshita: ""Photoresponsive behavior of self-assembling systems by amphiphilic alpha-helix with azobenzene unit"" Photochem.Photobiol., B : Biology. Vol.42, No.1. 143-150 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kishihara, T.Kinoshita, T.Mori and Y.Okahata: ""Vertical standing of Amphiphilic helical peptides at the hexane-water interface"" Chem.Lett.No.9. 951-952 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Ihara, N.Nakanishi, T.Sagawa, C.Hirayama, T.Sakurai, T.Kinoshita and Y.Tsujita: ""Detection of molecular-shape recognition for polycyclic aromatic hydrocarbons by alpha-helical poly (L-alanine) on silica"" Chem.Lett.No.10. 963-964 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kinoshita, T.Doi, A.Kato, Y.Tsujita and Yoshimizu: ""The guest-induced oscillation of a monolayer composed of polypeptide containing beta-cycldextrin at the terminal"" Chaos. Vol.9, No.2 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sugiyama, T.Sakurai, T.Yamamoto, T.Kinoshita, Y.Tsujita and H.Yoshimizu.: ""The Structure and Solubilization behaviors of Peptide-based Micelles"" Koubunshi Ronbun-shu. Vol.55 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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