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1998 Fiscal Year Final Research Report Summary

Molecular phylogeny of Rosaceae based on S-allele genotype and organelle DNA polymorphism

Research Project

Project/Area Number 09660021
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionGifu University

Principal Investigator

MATSUMOTO Shogo  Faculty of Education Gifu University Associate Prof., 教育学部, 助教授 (90241489)

Project Period (FY) 1997 – 1998
KeywordsRose / Apple / Molecular taxonomy / Self-incompatibility / matK / ITS / S-allele / PCR
Research Abstract

(1) Phylogenetic analysis among 32 Rosa species was carried out using the matK sequence data of organelle DNA.The molecular classification conformed closely to the traditional classification with some anomalies such as polyphyly of section Pimpinellifoliae. As six original species that contributed to modern roses were found to belong to only one clade, the genetic background of modem roses seems to be a narrow one.
(2) Phylogenetic analysis among 23 Rosa species was carried out using the ITS sequence data of nuclear DNA.The molecular classification was well in agreement with that of matK sequence analysis. Subgenus Platyrhodon and Hulthemia formed a clade within four subgenera.
(3) S-allele genotypes of 46 Malus x domestica cultivars and Malus species were identified by S-allele specific PCR analysis. This analysis was useful in determining the genetic relationship within the genus Malus.
(4) 'Tsugaru', 'Akagi', and 'Youkou' seem to have been produced by using pollen parents possessing the S_7-, S_7- or S_9-allele, respectively. It was suggested that there were at least three uncloned S-alleles. The S-genotypes of 'Kinsei' and 'Kizashi' were inconsistent with expected S-genotypes of their parents, indicating that they are derivatives from pollen parents having the S_9- or S_3-allele. A new S-allele, S_g, was proposed to be present in 'American Summer Pearmain', 'Indo', 'Kitanosachi', 'Shizuka', Mutsu' and 'Meku 10'.
(5) A new S-RNase, S_g, shows a high similarity to SS_f (94%) at the deduced amino acid level. There are only two amino-acid differences between HV regions, which are responsible for the S-allele specificity of SS_g and SS_f.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] S.Matsumoto: "Molecular classification of wild roses using organelle DNA probes." Scientia Horticulturae. 68. 191-196 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Matsumoto: "Chloroplast DNA probes as an aid in the molecular classification of Malus species." Scientia Horticulturae. 70. 81-86 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Matsumoto: "Phylogenetic analyses of the genus Rosa using the matK sequence : molecular evidence for the narrow genetic background of modern roses." Scientia Horticulturae. 77. 73-82 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Matsumoto: "S-genotypes of 15 apple cultivars and self-compatibility of ‘Megumi'" J.Japan Soc. Hort. Sci.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Matsumoto: "A new S-allele in apple, ‘Sg', and its similarity to the ‘Sf' allele in ‘Fuji'" HortScience. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Matsumoto: "Phylogenetic analyses of the subgenus Eurosa using the ITS nrDNA sequence." Acta Horticulturae. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Matsumoto, H.Wakita and H.Fukui: "Molecular classification of wild roses using organelle DNA probes." Scientia Horticulturae. 68. 191-196 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Matsumoto, H.Wakita and J.Soejima: "Chloroplast DNA probes as an aid in the molecular classification of Malus species." Scientia Horticulturae. 70. 81-86 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Matsumoto, M.Kouchi, J.Yabuki, M.Kusunoki, Y.Ueda and H.Fukui: "Phylogenetic analyses of the genus Rosa using the matK sequence : molecular evidence for the narrow genetic background of modern roses." Scientia Horticulturae. 77. 73-82 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Matsumoto, S.Komori, K.Kitahara, S.Imazu and J.Soejima: "S-genotypes of 15 apple cultivars and self-compatibility of 'Megumi'." J.Japan Soc.Hort.Sci.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Matsumoto, K.Kitahara, S.Komori and J.Soejima: "S-allele in apple, 'Sg', and its similarity to the 'Sf' allele in 'Fuji'." A new HortScience.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Matsumoto, M.Kouchi, Y.Ueda and H.Fukui: "analyses of the subgenus Eurosa using the ITS mDNA sequence." Phylogenetic Acta Horticulturae.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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