1998 Fiscal Year Final Research Report Summary
Wolbachia infection and reproductive incompatibility in spider mites
| Project/Area Number |
09660040
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | IBARAKI UNIVERSITY |
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Principal Investigator |
GOTOH Tetsuo IBARAKI Univ.Fuc.of Agr, Associate Prof., 農学部, 助教授 (60178449)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Wolbachia / reproductive incompatibility / Tetranychus urticae / Panonychus mori / Tetranychus kanzawai |
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| Research Abstract |
Wolbachia infection in 326 populations (including 10 populations from abroad) of 20 mite species was assayed by the polymerase chain reaction (PCR), using the 16S rDNA and the ftsZ primers which amplify 890-bp and 770-bp segment, respectively. Wolbachia were detected in 55 populations of 4 species : i.e., the green and red forms of Tetranychus urticae, T.kanzawai, T.pueraricola and Panonychus mori. Of them, Wolbachia that infects four populations of P.mori was related to reproductive incompatibility. Other populations appeared to harbor a neutral strain of Wolbachia that does not cause incompatibility. It is likely that infection with a neutral strain is not unusual in mites.
Out of five populations of P.mori that infected with Wolbachia, the Tsuruoka (TR), Toyama (TY), Hanayama (HN) and Haruno (HR) populations harbored Wolbachia that mediates reproductive incompatibility, while the Sendai (SD) population infected with a neutral strain. For example, unidirectional incompatibility has been observed m crosses between the Wolbachia-free Ami (AM) population and either of the TR or TY populations. As crosses between females from the AM population and rifampicin-treated males from either of the TR or TY populations restored compatibility, Wolbachia could have been responsible for the incompatibility.
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