1998 Fiscal Year Final Research Report Summary
Studies on location of xenobiotics degradation in the cell of BHC-degrading bacterium.
| Project/Area Number |
09660073
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAGATA Yuji The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant Professor, 大学院・農学生命科学研究科, 助手 (30237531)
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| Project Period (FY) |
1997 – 1998
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| Keywords | BHC / Xenobiotics / Sphingomonas / Subcellular localization / Periplasm |
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| Research Abstract |
gamma-Hexachlorocyclohexane (gamma-HCH, BHC, and lindane) is one of several highly chlorinated insecticides that cause serious environmental problems. The cellular proteins of a gamma-HCH-degrading bacterium Sphingomonas paucimobilis UT26 were fractionated into periplasmic, cytosolic and membrane fractions after osmotic shock. Most of activities of LinA, LinB, LinC, LinD, and LinE that are involved in the early steps of gamma-HCH degradation in UT26, were detected in the periplasmic fraction. Antibodies were raised against LinA and LinB.Most of the LinA and LinB protein was detected in the periplasmic fraction and had not undertaken molecular processing. Furthermore, immunoelectron microscopy clearly showed that LinA and LinB are periplasmic proteins. LinA and LinB both lack a typical signal sequence for export, so they may be secreted into the periplasmic space via a hitherto unknown mechanism.
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