1998 Fiscal Year Final Research Report Summary
Utilization of Microbial Cellodextrin and Cellobiose Phosphorylase
| Project/Area Number |
09660101
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
ARAI Motoo College of Agriculture, Osaka Prefecture University Professor, 農学部, 教授 (80081537)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Phosphorylase / Cellobiose / Cellodextrin / Clostridium |
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| Research Abstract |
The genes of cellobiose phosphorylase and cellodextrin phosphorylase were cloned and these genes expressed effectively in Eschrichia coli. These enzymes were obtained easily from E. coli cells. Using these enzyme, the cellooligosaccharides whose reducing end was p-nitrophenol or 14C-labeled glucose were synthesized. These oligosaccharides was effective tool to elucidate of mechanism of cellulose hydrolysis by cellulases.
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