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1998 Fiscal Year Final Research Report Summary

Studies on regulation of cell growth of budding yeast by platelet-activating factor (PAF)

Research Project

Project/Area Number 09660104
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionKyoto Women's University

Principal Investigator

NAKAYAMA Reiko  Kyoto Women's University, Department of Food Science and Nutrition, Associate Professor, 家政学部, 助教授 (60172483)

Project Period (FY) 1997 – 1998
KeywordsYeast / Saccharomyces cerevisiae / Platelet-activating factor (PAF) / Cell growth / Mating process / Lipid mediator
Research Abstract

The purpose of this investigation was to define the possible function of PAF (platelet-ctivating factor) in budding yeast in relation to cell growth regulation.
1.Changes in PAF Production and Distribution of S.cerevisiae cells in Various phase of Life Cycle
We examined the changes of PAF production and its cellular distribution in S.cerevisiae during cell cycle. PAF production of cells was the highest at G_1 phase, and 90 % of PAF was localized in the cell surface. And, we found that the yeast produces PAF in early stage of mating process in response to the mating hormone. Furthermore, STE6 gene disruptant (MAT a cell) was made by using ste6 : URA3, and PAF production and distribution of the disruptant were compared with those of wild type cells. Results indicated that Ste6p encoded by STE6 gene, transport not only lipopeptide mating hormone a-factor, but also lipid mediator PAF.
2.Mechanism of Regulation of Cell Growth by PAF
PAF was added to culture medium and yeast cell growth was monitored. PAF inhibited cell growth dose-ependent manner. We obtained PAP-insensitive mutants, and examined the effects of PAF on the concentration of intracellular cAMP, activities of membrane adenylate cyclase and metabolism of phosphatidyl inositol (cellular IP_3). Results showed that mechanisms of PAF-induced inhibition of cell growth were related to RAS-cAMP pathway and/or the signal transduction mediated by IP_3.
3.Cloning of PAF-ensitive Gene
PAF-sensitive gene, GPR1 was isolated. Gprlp, encoded by GPR1 gene was not PAF receptor, but it is aG-protein coupled receptor which localized at plasma membrane, and regulates the cellular cAMP level in response to the extracellular signal such as glucose.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Reiko Nakayama: "Changes in PAF(platelet-activating factor) production during cell cycle of yeast S.cerevisiae" Biosci.Biochem.Biophys.61. 631-635 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.W.Yun: "G-protein coupled receptor from yeast Saccharomyces cerevisiae" Biochem.Biophys.Res.Commun.240. 287-292 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中山 玲子: "出芽酵母はなぜ血小板活性化因子を産生するのか?" バイオサイエンスとインダストリー. 56. 254-256 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.W.Yun: "Gprlp, a putative G-protein coupled receptor, regulates glucose dependent cellular cAMP level in yeast Saccharomyces cerevisiae" Biochem.Biophys.Res.Commun.252. 29-33 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reiko Nakayama, H.Udagawa and H.Kumagai: "Changes in PAF (platelet-activating factor) production during cell cycle of yeast S.cerevisiae" Biosci.Biochem.Biophys.61. 631-635 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Cheolwon Yun, H.Tamaki, R.Nakayama, K.Yamamoto and H.Kumagai: "G-protein coupled receptor from yeast Saccharomyces cerevisiae" Biochem.Biophys.Res.Commun.240. 287-292 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Reiko Nakayama: "Why do budding yeast cells produce platelet-activating factor?" Bioscience and Industries, (Baiosaiennsu to Inndasutori, Japanese). 56. 254-256 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Cheolwon Yun, H.Tamaki, R.Nakayama, K.Yamamoto and H.Kumagai: "Gpr1p, a putative G-protein coupled receptor, regulates glucose dependent cellular cAMP level in yeast Saccharomyces cerevisiae" Biochem.Biophys.Res.Commun.252. 29-33 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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