• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1998 Fiscal Year Final Research Report Summary

Screening of new appatite regulating factors

Research Project

Project/Area Number 09660132
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionThe University of Tokyo

Principal Investigator

HIDAKA Tomomi  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (60212164)

Project Period (FY) 1997 – 1998
Keywordsleptin / appetite regulation / Ca^<2+> / calmodulin / NPY / biosensor
Research Abstract

To understand the leptin's action on hypothalamic arcuate nucleus (ARC), Ca^<2+>/calmodulin (CAM) -dependent response in leptin-treated rat ARC was investigated. The in vitro phosphorylation of ARC extracts from leptin-treated rats did not respond to CaM.Although CaM-dependent protein kinase ll activity was not affected by leptin, the gene expression of calcineurin, CaM-dependent phosphatase, increased in leptin-treated rats.
In the purpose of getting tools for understanding the leptin's biological actions, low molecular weight microbial metabolites, which interact with leptin, were screened using a BlAcore instrument (BlAcore2000, Biacore), the surface plasmon resonance sensor. As a results, linoleic acid was isolated as a active compound. It was revealed that the double bond and the carboxyl group of fatty acids are essential for the binding affinity.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 日高智美: "急速に展開する肥満遺伝子研究" 日本栄養・食糧学会誌. 50. 87-89 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takehiko Morishita: "Leptin changes Ca^<2+>/calmodulin-dependent response and up-regulates the gene expression of calcineurin in rat hypothalamus" Life Sciences. 63. PL311-315 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 日高智美: "レプチン研究の新しい可能性" バイオサイエンスとバイオインダストリー. 56. 37-38 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomomi Hidaka: "Screening of microbiol products modifying the action of leptin by a biosensor" Journal of Antibiotics. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Morishita, T.Hidaka, K.Sugahara, T.Noguchi: "Leptin changes Ca^<2+>/calmodulin-dependent response and up-regulates the gene expression of calcineurin in rat hypothalamus" Life Sciences. 63. PL311-315 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Hidaka, T.Morishita, K.W.Shimothono, T.Endoh, H.Seto and T.Noguch: "Screening of microbial products modifying the action of leptin (obese gene product) by a biosensor." J.Antibiotics.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-12-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi