1999 Fiscal Year Final Research Report Summary
Study on the light-protecting effect of polyacylated anthocyanins
| Project/Area Number |
09660147
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食品科学・栄養科学
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| Research Institution | Sugiyama Jogakuen University |
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Principal Investigator |
KAMEDA Kiyoshi School of Life Studies, Professor, 生活科学部, 教授 (60104626)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Kumi School of Life Studies, Assistant Professor, 生活科学部, 助手 (90210690)
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| Project Period (FY) |
1997 – 1999
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| Keywords | heavenly blue anthocyanin / polyacylated anthocyanin / intramolerular stacking ] / UV-B / DNA damage / Z-caffeoyl residue / light-protecting effect / Okazaki large spectrography |
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| Research Abstract |
The antohcyanin is the natural colorant which is not only a safe food-colorant but also is expected antioxidant, cancer inhibition, and immunostimulating effect and, etc. In the meantime, ultraviolet ray protecting function can be estimated because the genetic hazard does not occur, even if the flower, a genital organ, blooms under the solar light for a long period. This function is very interesting for basic science study, and in addition, it is important from the viewpoint of the development of the highly functional food-coloring agent. For this purpose I studied on stability of heavenly blue anthocyanin (HBA) from Ipomoea tricolor and protection of DNA damage.
1. Light irradiating experiment using the Okazaki Large Spectrograph (OLS).
HBA was dissolved in 0.5% TFA-methanol solution, or pH4, 6, and pH 7.5 buffer solution, then using the OLS, monochromatic UV light from 280 nm to 400 nm was irradiated. Under the physiological condition, HBA was the most stable, and in acidic methanol the most unstable. The pigment decomposed more rapidly at 280 nm than 370 nm.
2. A protection of DNA damage by the pigment.
HBA and bovine thymus DNA was dissolved in pH7.5 buffer solution, and then the solution was irradiated by ultraviolet radiation of UV-B region. The degree of a DNA damage was determined quantitatively by the ELISA in the cooperation of Professor Nikaido of the Kanazawa University. HBA exhibited the protecting effect against degradation of DNA by UV-B irradiation.
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[Publications] Fukui,Y., Kusumi,T., Yoshida,K., Kondo,T., Matusda,C., Nomoto,K.: "Structures of two diacylated anthocyanins from Petunia hybrida cv. surfinia violet mini."Phytochemistry. 47. 1409-1416 (1998)
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