1999 Fiscal Year Final Research Report Summary
Research storage light environment for quality preservation of micropropagated transplants
| Project/Area Number |
09660280
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物環境
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| Research Institution | Chiba University |
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Principal Investigator |
KUBOTA Chieri Faculty of Horticulture, Chiba University, Associate Professor, 園芸学部, 助教授 (00280917)
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| Co-Investigator(Kenkyū-buntansha) |
全 昶厚 千葉大学, 園芸学部, 助手
KOZAI Toyoki Faculty of Horticulture, Chiba University, Professor, 園芸学部, 教授 (90081570)
CHUN Changhoo Faculty of Horticulture, Chiba University, Assistant Professor
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| Project Period (FY) |
1997 – 1999
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| Keywords | low temperature storage / plug transplants / light quality / light compensatin point / transplant production / photosynthesis |
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| Research Abstract |
The investigators have been studied on the effects of light intensity, air temperature, photoperiod, and light quality during storage on the growth and quality of the plantlets. One of the important findings is that plantlets respond to light quality even under low temperature conditions. We have found a positive effect of green light for maintaining photosynthetic ability of stored plug plantlets that have a dense canopy. According to data obtained in the first two years on the plantlet responses to light and temperature environments in storage, we have developed mathematical models to predict the dry weight changes during storage as affected by storage environmental conditions. The predicted dry weight changes during storage were well fitted to the measured values under given combinations of air temperature and photosynthetic photon flux. Use of modeling and simulation techniques was shown to be effective for determining storage environmental conditions and for duration of storage.
Using tomato and sweet potato plantlets micropropagated in vitro, we have also examined the effects of sugar concentration on the carbohydrate concentrations of plantlet, since carbohydrate status of the plantlets is a critical factor for storage. Papers are now under preparation for publishing in scientific journals with peer review process.
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[Publications] Willson,S.B., C.Kubota, J.W.Heo, N.C.Rajapakse, R.E.Yong and T.Kozai: "Carbohydrate status of micropropagation photoautotorophic and photomixotrophic plantlets"Abstr.of Intl. Symp.on transplant Production in Closed System for Solving the Global Issues on Environmental Conservation, Food, Resource and Energy, Feb. 28-Mar. 2,2000,Chiba, Japan. 22 (2000)
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