1998 Fiscal Year Final Research Report Summary
Potencial of fermented milk for anti-adhesion therapy
| Project/Area Number |
09660284
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Zootechnical science/Grassland science
|
|---|
| Research Institution | Hirosaki University |
|---|
Principal Investigator |
TOBA Takahiro Hirosaki University Faculty of Agriculture and Life Science Associate Professor, 農学生命科学部, 助教授 (10108483)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Keywords | Lactobacillus crispatus / S-layer protein / gene / amino acid sequence / adhesion / anti-adhesion therapy / laminin / enetotoxigenic Escherichia coli |
|---|
| Research Abstract |
Before this project had started, we had shown that Lactobacillus crispatus JCM 5810 well adhered to submucosal model and its 43-kDa S-layer protein(CbsA) had been identified as an adhesin.
In the year 1997 primary structure of CbsA was determined by DNA sequencing. Alignment of CbsA and S-layer protein(SIpA) of non-adhesive Lactobacillus acidophilus 1CM 1132(=ATCC 4356) indicated that signal sequence of 30 amino acids was identical except one residue. The identity of two proteins was 77% in C-terminal sequence and 30% in N-terminal region. These results suggested binding region might be located in the N-terminal region.
In the year 1998 deletion and point mutation was incorporated into CbsA gene and the expressed His-tag fusion protein was evaluated for binding to type IV collagen. The results suggested that 1-2 87 amino acid sequence was necessary to bind type IV collagen. Lb. crispatus JCM 5810 and its S-layer protein inhibited the adherence of enterotoxigenic Escherichia coli on Matrigel and immobilized laminin, used as an submucosal model.
The above results suggested S-layer-bearing lactobacillar strains and their S-layer protein have the potential to apply anti-adhesion theraputic agent for intestinal infection disease to inhibite collonization of pathogens.
|
