1998 Fiscal Year Final Research Report Summary
A SIMPLE METHOD FOR THE DETECTION OF THE TRANSPORT PATHWAYS OF MACROMOLECULES ACROSS ARTERIAL ENDOTHELIUM
| Project/Area Number |
09660313
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | IWATE UNIVERSITY |
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Principal Investigator |
TANIGUCHI Kazuyuki FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (70148089)
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| Co-Investigator(Kenkyū-buntansha) |
TAIRA Hideharu FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (70045756)
OGAWA Kazushige FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR (60231221)
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| Project Period (FY) |
1997 – 1998
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| Keywords | ENDOTHELIUM / CAVEOLAE / VESICLES / INTERCELLULAR CLEFTS / NEM / CHANNELS / PERMEABILITY / BLOOD VESSEL |
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| Research Abstract |
Serum macromolecules are transported through the vascular endothelial layer to the interstitium via the caveolae and interendothelial clefts, but nature of the permeability of these structures is unknown, and the manner of caveola-vesicle transport is controversial, We have developed an elegant method of detecting macromolecular channels using an in situ tracer perfusion after fixation and random conventional sectioning for electron microscopy. We observed the caveolar and intercellular channels through the endothelial layer, and only a few population of vesicles free in the cytoplasm except those around the Golgi area of the arterial endothelium. Using unbiased morphometry, 5% of the abluminal caveolae and 15% of the intercellular clefts formed transendothelial channels. In rat aortas treated with N-ethylmaleimide, intercellular channels formed de novo at a frequency more than six times that of the physiological controls ; the density of the abluminal caveolae was decreased to 80% of the physiological control level while the larger invaginations increased in the number. We therefore propose that caveolar channels rather than transcytosis provide a mechanism of caveola-vesicle transport in the endothelium, because transcytosis involves many free vesicles, and that a flexible channel system functions extensively as a macromolecular transport pathway in the arterial endothelium.
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