1998 Fiscal Year Final Research Report Summary
Organculture as a new model of atherosclerosis
| Project/Area Number |
09660315
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OZAKI Hiroshi The University of Tokyo, Graduate School od agricultural and life sciences, Associate professor, 大学院・農学生命科学研究科, 助教授 (30134505)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Koichi The University of Tokyo, Graduate School od agricultural and life sciences, Assi, 大学院・農学生命科学研究科, 助手 (90205914)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Vascular smooth muscle / PDGF / Fetal bovine serum / interleukin-IB / organ culfure / 動脈硬化 |
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| Research Abstract |
The purpose of this study is to develop new experimental model of a vascular atherosclerosis. For this purpose, we utilized a vascular organ culture system, rather than cultured endothelial cells, which has the advantages to obtain the conditions that better preserves tissue architecture, cell-to-cell interactions, the extracellular matrix, and the morphology and function of differentiated cells and thereby to reproducibly study behavior of the whole tissue in response to environmental stimuli. We tested the effect of tnree agents, interleukin-1B, fetal bovine serum (FBS) ana platelet derived growth factor (PDGF), which are assumed to be related to the vascular growth related disease. The results obtained are follows.
1) We first demonstrated that Interleukin-1B relaxes vascular smooth muscle by the NO-dependent and independent mechanisms (Takizawa et aI. : Eur. J.Pharmacol. 330 : 143-150, 1997). As for the NO-independent mechanism, we further revealed that membrane hyperpolarization due to activation of ATP-sensitive K channels may partly be responsible for it.
2) Chronic treatment with FBS markedly decreased the endothelial NO-dependent relaxation in rabbit mesenteric artery. Total mRNA for endothelial NO synthase were also reduced. PDGF showed similar results with FBS.These results suggest that growth activation of rabbit mesenteric artery decreases endothelial NO synthase mRNA, reduces NO production and impairs endothelium-dependent relaxation.
These results suggest that organ culture is a useful method for studying the mechanism of
vascular growth related disease.
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[Publications] Karai, H., Ozaki, H., Hori, M., Mitsui-Saito, M., Amano, K., Harada, K., Miyamoto, S., Nakazawa, H., Won, K.-J.and Sato, K.: "Calcium movements, distribution, and functions in smooth muscle." Pharmacol.Rev.49. 157-230 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamawaki, H., Sato, K., Hori, M., Ozaki, H., Nakamura, S., Nakayama, H., Doi, K.and Karaki, H.: "Inpairment of endothelium dependent relaxation following incubation of arteries with FBS." Eur.J.Pharmacol.366. 237-242 (1999)
Description
「研究成果報告書概要(欧文)」より
