1999 Fiscal Year Final Research Report Summary
Expression of liver specific functions in chicken hepatocytes
| Project/Area Number |
09660322
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Kagoshima University |
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Principal Investigator |
FUJII Makoto Kagoshima University, Agriculture, Professor, 農学部, 教授 (70041655)
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| Co-Investigator(Kenkyū-buntansha) |
HOU De-xing Kagoshima University, Agriculture, Associate Professor, 農学部, 助教授 (90305160)
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| Project Period (FY) |
1997 – 1999
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| Keywords | Chicken / Hepatocyte / Primary culture / Serum-free culture / Liver specific function / Depoisoning enzymes / Environmental pollution chemicals |
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| Research Abstract |
Rats primary hepatocytes have been usually cultured in a 5% COィイD22ィエD2 incubator at pH 7.4. However, we found that chicken hepatocytes were able to cultured in serum-free L-15 medium for a long period in free gas exchange with the atmosphere (without COィイD22ィエD2 gas). The amount of albumin secreted in the medium decreased to low level early, followed by marked increase from day 9 to day 17 of culture. The profile of appearance of albumin m-RNA was very similar to that of albumin protein. Such observation was not observed in rat hepatocytes, showing some difference in the properties of hepatocytes between avian and rat. Therefore, we tried to monitored the effects of enbironmental pollution chemicals on metabolism in liver of wild birds using chicken hepatocytes as a model. 10ィイD1-1ィエD1 M TCDD (tetracholoro-dibenzo-p-dioxin) decreased the expression of albumin, and 10ィイD1-2ィエD1 M TCDD markedly induced the expression of the depoisoning enzyme, P450 CYP1A4.
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[Publications] Fujii M., Yoshino I.., Suzuki M., Higuchi T., Mukai S. ,Aoki T. , Fukunaga T. , Sugimoto Y. , Inoue Y. , Kusuda J. , Saeki T . , Sato M. , Hayashi S. ,Tamaki M. , Sugano T.: "Primary culture of chicken hepatocytes in serum-free medium(pH 7.8) secreted albumin and transferrin for a long atmosphere."Int. J. Biochem. Cell Biol.. 28. 1381-1391 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Fujii M.,Yonekura M.,Higuchi T., Morimitsu K.,Yoshino I., Mukai S., Aoki T., Fukunaga T., Inoue Y., Sato M., Kanaeda J.: "Effect of 350kDa glycoprotein in royal jelly on primary culture of rat hepatocytes"Food Sci. Technol. Int.. 2. 223-225 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Fujii M., Suzuki M., Yoshino I., Higuchi T., Manaka M., Aoki T., Fukunaga T., Kusuda JH ., Saeki T., Sugimoto Y., Sugano T., Tamaki M.: "An economical method for primary culture of chicken hepatocytes in serum-free medium"Jpn. Poult. Sci.. 33. 15-22 (1996)
Description
「研究成果報告書概要(欧文)」より
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