1999 Fiscal Year Final Research Report Summary
Genome analysis of T.equigenitalis and the moleculu epidemiology
| Project/Area Number |
09660333
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Azabu University |
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Principal Investigator |
MATSUDA Motoo College of Environmental Health Science, Azabu University, Professor, 環境保健学部, 教授 (50139531)
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| Project Period (FY) |
1997 – 1999
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| Keywords | Taylorella-equigenitalis / contagious equine / motutis / pulsed-field gel electrophoresis / restriction enzymes / genome analysis / molecular epidemiology |
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| Research Abstract |
Contagious equine metritis (CEM) is a sexually transmitted bacterial disease of horses caused by Taylorella equigenitalis, a Gram-negative eubacterium. This species was only relatively recently identified. The GEM was first described in England in 1977. Since then it has been detected in many countries, namely Ireland, the USA, Australia, Europe and Japan and in various horses.
The first occurrence of CEM in horses in Japan and the isolation of T.equigenitalis from cervical swabs from mares with endometritis were reported in 1980.
No attempts have been made to biologically and/or serologically type T.equigenitalis so far we are aware before we have started this research project. It would be very important to clarify scientifically the mechanism(s) of occurrence and route(s) of transmission for CEM and the causative agent, T. equigenitalis.
In the present study we analyzed the restricted genomic DNAs from one hundred and several ten isolates of T. equigenitalis isolated from Ireland, the USA, Australia, England, Norway and Japan by pulsed-field gel electrophoresis(PFGE) after separate digestion with ApaI and NotI.
Consequently, ten and several genotypes were detected among strains of T. equigenitalis examined and a Japanese common genotype J for all strains isolated in Japan was for the first time found among a strain in England.
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Research Products
(11 results)
