1998 Fiscal Year Final Research Report Summary
Pathobiology of leukocyte functions from cattle with leukocyte adhesion deficiency(BLAD)and trials of bone marrow transplantation to BLAD-cattle.
| Project/Area Number |
09660344
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | RAKUNO GAKUEN UNIVERSITY,FACULTY OF VETERINARY MEDICINE |
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Principal Investigator |
NAGAHATA Hajime RAKUNO GAKUEN UNIVERSITY,SCHOOL OF VETERINARY MEDICINE,PROFESSOR, 獣医学部, 教授 (10133571)
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| Co-Investigator(Kenkyū-buntansha) |
KUWABARA Mikinori GRADUATE SCHOOL OF VETERINARY MEDICINE,HOKKAIDO UNIVERSITY,PROFESSOR, 大学院・獣医学研究科, 教授 (10002081)
INANAMI Osamu GRADUATE SCHOOL OF VETERINARY MEDICINE,HOKKAIDO UNIVERSITY,ASSOC.PROFESSOR, 大学院・獣医学研究, 助教授 (10193559)
TERAOKA Hiroki RAKUNO GAKUEN UNIVERSITY,FACULTY OF VETERINARY MEDICINE,ASSIST.PROFESSOR, 獣医学部, 講師 (50222146)
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| Project Period (FY) |
1997 – 1998
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| Keywords | BLAD TRANSFUSION / BONE MARROW TRANSPLANTATION / CD18 / GENETIC DISEASE / TRANSFUSION / CATTLE DISEASE |
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| Research Abstract |
The above-entitled study was performed and the results were summarized as follows :
Fc receptor for immunoglobulin G-mediated phagocytosis, superoxide production and intracellular calcium ([Ca^2+]^i) signaling of complement receptor type 3-(CR3) deficient neutrophils from a heifer with leukocyte adhesion deficiency were compared to those of control heifers. The mean phagocytic activity of IgG-coated yeasts and aggregated bovine IgG (Agg-IgG)-induced superoxide production of CR3-deficient neutrophils were 10% and 77.9%, respectively, of those of control neutrophils. The [Ca^2+]^i signals in CR3-deficient neutrophils stimulated with Agg-Igg or concanavalin A were deficient with mean peak [Ca^2+]^i concentrations of 78% and 42% of those of controls. These findings suggest that Fc receptor-mediated neutrophil functions are closely dependent on the presence of CR3 on the neutrophil cell surfaces.
The emigration of leukocytes from calves with B2 integrin deficiency (BLAD) into bronchoalveolar
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spaces and scraped tissues was compared to that of normal calves. Neutrophils were found in bronchoalveolar lavage fluid from BLAD-affected calves showing chronic -pneumonia. The neutrophils were CR3-negative when characterized by flow cytometric analysis using anti-CD18 monoclonal antibody. Chemiluminescent response mediated by CR3 in neutrophils isolated from bronchoalveolar lavage fluid from BLAD-calves showed similar findings obtained from CR3-deficient neutrophils. Neutrophils from normal calves migrated to the scraped tissue, evaluated by skin window method. These findings confirmed the extravasating property of normal and CR3-deficient neutrophils into scraped tissues.
Granulocyte transfusion as performed in an 8-month-old heifer with BLAD to monitor the changes in transfused CDl8-positive neutrophils and associated neutrophil chemilurninescent response in B2 integrin deficient host. The CD18-positive neutrophils were detected in blood from the BLAD heifer during the first 3 hr after 2.6xl0^9cells were infused by GT, and disappeared by 5 hr after GT.The CL response of neutrophils was increased 1.7 to 2.8-fold in the BLAD heifer during the first 3 hr after GT, thereafter CL response decreased gradually from 2 to 5 hr after GT.
Bone marrow transplantation (BMT) was performed in a 9-month-old heifer with BLAD.Clinical and hematological findings, selected neutrophil function and CD18 expression of neutrophils in a B2 integrin-deficient heifer were examined. Twelve months after BMT, a small fluorescent region in the CD18-positive area of a flow cytometric profile was demonstrated and estimated to represent 0.3-0.5% of the CD18-positive neutrophils as measured by flow cytometric analysis following irnmunomagnetic separation. The animal's clinical condition appeared to improve and stabilize over our observation period of 28 months following BMT.Newly expressed CD18 seemed to play an important role in ameliorating the clinical signs of BLAD in this heifer. Less
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Research Products
(12 results)
