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1999 Fiscal Year Final Research Report Summary

How does the inhibin family relate to the development of the granulosa cell tumors?

Research Project

Project/Area Number 09660345
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionAzabu University (1998-1999)
Kitasato University (1997)

Principal Investigator

MADARAME Hiroo  Azabu university Associate Professor, 獣医学部・附属動物病院, 助教授 (20173768)

Co-Investigator(Kenkyū-buntansha) OGATA Munetsugu  , 助教授 (10063968)
YOSHIDA Shintaro  Kitasato university Assistant Professor, 獣医畜産学部・実験動物学教室, 助手 (40286365)
HASEGAWA Yoshihisa  Kitasato university Professor, 獣医畜産学部・実験動物学教室, 教授 (40092001)
Project Period (FY) 1997 – 1999
KeywordsInhibin family / Granulosa cell tumor / Inhibin / Activin / Follistatin
Research Abstract

1. The implantation of ovarian tissue into the splenic capsule of gonadectomized female rats developed into benign granulosa cell tumors between 6, 8, and 10 months after autotransplantation. We investigated the immunohistochemistry of inhibin, activin, and follistatin in the implanted ovary, and the serial serum assay of inhibin, luteinizing hormone(LH), and follicle-stimulating hormone(FSH). The serial serum hormone assay revealed that the concentration of serum inhibin and FSH had an inverse relationship. The serum FSH level raised rapidly after implantation and this high level was sustained for at least 120 days after operation. Then, the marked increase of inhibin concentration was accompanied by a sudden decrease of FSH concentration. In contrast, LH level fluctuated in all cases. In immunostaining of inhibin, a high percentage of the tumor cells revealed an intensive fine to coarse granular positive reaction in the cytoplasm. In contrast to inhibin, activin and follistatin immunostaining was not identified in tumor cells. In conclusion, granulosa cell tumors secreted inhibin, and serum inhibin level elevated. Inhibin can be used as a histopathological and serological marked of granulosa cell tumors.
2. In hypogonadal mouse(hpg mouse), the implantation of ovarian tissue into the splenic capsule of gonadectomized female mice could not develop into any tumors. Further investigation should be required, but it would be difficult to develop granulosa cell tumor in hpg mice.
3. In domestic animals, mainly in dogs, the immunostaining of inhibin revealed various positive reactions in the granulosa cell tumors. Inhibin can be used as a histopathological marked for granulosa cell tumors.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Madarame,H.: "Canine Mesenchymal Chondrosarcoma of the Ribs"J. Vet. Med. Sci.. 60. 975-979 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Madarame,H.: "Pathogenicity of R. equi strains Possessing Virulence-associated 15 to 17-kDa and 20-kDa Antigens"J. Comp. Pathol.. 119. 397-405 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Madarame,H.: "Cutaneous malakoplakia : experimental R. equi infection in pigs"FEMS Innunol. Med. Microbiol.. 22. 329-333 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasaki,Y.: "The alkaline single cell gel electrophoresis assay with mouse multiple organs"Mut. Res.. 440. 1-18 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kasuga-Aoki,H.: "TNF and Interferonγ are required in host resistance against virulent R. equi infection in mice"Immunol.. 96. 122-127 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hasegawa: "Inhibins, activins and follistatins Novel regulators for follicle development"Proceeding of International Workshop on Embryogenesis and Implantation. (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hasegawa: "Inhibin, Activin and Follistatin"Springer-Verlag. 151-161 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Madarame,H.: "Virulent and avirulent Rhodococcus equi infection in T-cell deficient athymic nude mice : Pathologic, bacteriologic and immunologic responses."FEMS Immunology and Medical Microbiology. 17. 251-262 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Madarame,H., Y.Canine: "Canine Mesenchymal Chondrosarcoma of the Ribs."Journal of Veterinary Mededical Science. 60. 975-979 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Madarame,H.: "Pathogenicity of Rhodococcus equi Strains Possessing Virulence-associated 15-to17-kDa and 20-kDa Antigens : Experimental and Natural Cases in Pigs."Journal of Comparative Pathology. 119. 397-405 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Madarame,H.: "Cutaneous malakoplakia : experimental Rhodococcus equi infection in pigs."FEMS Immunology and Medical Microbiology. 22. 329-333 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasaki,Y.: "The alkaline single cell gel electrophoresis assay with mouse multiple organs : Results with 30 aromatic amines evaluated by the IARC and U.S.NTP."Mutation Research. 440. 1-18 (199)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kasuga-Aoki,H.: "Tumor necrosis factor and interferon-γ are required in host resistance against virulent Rhodococcus equi infection in mice : cytokine production depends on the virulence levels of R.equi."Immunology. 96. 122-127 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurusu,S.: "Cytosolic phospholipase AィイD22ィエD2 in rat decidual cells : evidence for its role in decidualization."FEBS Letters. 444. 235-238 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Davis,W.P.: "Rhodococcus equi infection in two nubian-cross goats."Veterinary Pathology. 36. 336-339 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hasegawa,Y., kawarasaki,S., and Madarame,H.: "Inhibins, activins and follistatins ; Novel regulators for the follicle development and oocyte growth."in ; Proceeding of International Workshop on Embryogenesis and Implantation. in press. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hasegawa,Y.: "Two-site immunoassay for native inhibin A in "Inhibin, Activin, and Follistatin.", 1997, Eds.Aono T, Sugino H and Walw W"Springer-Verlag, New York. 151-161

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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