1998 Fiscal Year Final Research Report Summary
Mechanism for a dendritic cell transport in the hepatic sinusoids and lymph.
| Project/Area Number |
09670019
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | KUMAMOTO UNIVERSITY |
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Principal Investigator |
MATSUNO Kenjiro Kumamoto Univ.School of Medicine, Lecturer, 医学部, 講師 (20094047)
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| Co-Investigator(Kenkyū-buntansha) |
EZAKI Taichi Kumamoto Univ.Medicine, Research Assistant, 医学部, 助手 (10128259)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Liver / Hepatic Sinusoids / Hepatic Lymph / Dendritic Cells / Migratory Pathway / Kupffer Cells / Cell Adhesion Molecules |
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| Research Abstract |
Aim of this study : Liver is constantly surveyed by a traffic of antigen-presenting dendritic cells (DC). We studied about the transport system of DC within the liver microarehitecture and factors which regulate this transport.
Result 1. intravenously transferrd rat DC translocated and selectively accumulated in the paracortex of the hepatic lymph nodes (published). Soon after the rat liver transplantation, donor MHCII+ cells migrated to both T and B cell areas of thc spleen. The hepatic lymph leaked into the peritoncal cavity and DC in lymph were absorbed into diaphragmatic lymph plexus and transported to the anterior mediastinal LN.Donor MHCII+ cells arc speculated to correspond to DC progenitors in the liver (in preparation).
Result 2. A large number of rat DC were injected intravenously to allogencic host and a fate of the DC in the liver tissue was traced lime kinetically. We found that DC were first entrapped by the Kupffer cells in the sinusoidal wall possibly through mechanical and molecular interactions. The entrapped DC may extravasate into the Disse's space and then exit the hepatic lobule into tissue space of the portal area. Eventually these cells probably crawl into the lymphatic capillary located in the portal area (presented at 103rd Japanese Anatomy Congress). This will be confinned by in vivo observation using confocal laser microlluorography by a collaboration with Dr. M.Suematsu, Keio University (now under study).
Result 3. In the sinusoidal area a close association between the DC and Kupffer cells is often observed in normal rats. Rat Kupffer cells in the hepatie sinusoids are capable of selectively trapping DC from the blood in vivo and in vitro (published) suggesting a presence of certain adhesion molecules, possibly sugar residues and leetins. By this, DC could attach to the sinusoidal wail probably as a prelude to extravasation. This is now under study by collaborating with Dr. T.Suganuma. Miyazaki Medical College.
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