1999 Fiscal Year Final Research Report Summary
The role of actin microfilament cytoskeleton on the cell adhesion of chick embryonic arterial endothelium
| Project/Area Number |
09670036
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Gunma prefectural College of Health Sciences |
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Principal Investigator |
JINGUJI Yoichi Dept of Nursing, Gunma prefectural College of Health Sciences, Professor, 看護学科, 教授 (00114182)
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| Project Period (FY) |
1997 – 1999
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| Keywords | chick embryo / actin microfilament / stress fiber / cytochalasin D / endothelium / cultured endothelial cell |
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| Research Abstract |
This project aimed to reveal the distribution pattern of actin microfilaments especially of the stress fibers in the developing chick embryonic arterial endothelium. The immunofluorescence and electron microscopy, including laser scanning confocal microscopy were used in this study.
Arterial endothelial cells of chick embryos aged over 9 days of incubation have well developed stress fibers oriented parallel to the major axis of the cell.
When 0.1 ml of 20 μg/ml cytochalasin D was injected into the arterial branch of chorio-allantoic membrane of 15 to 16 day-old chick embryos, the distribution of stress fibers decreased within one hour. This change was accompanied by the appearance of a distinct bundle of actin microfilaments at the cell border. These actin bundles may support the cell to cell adhesion at the cell periphery where the adrehens junction may present. Some of the remaining stress fibers have a periodic banding pattern with rhodamine-phalloidin staining. Similar sarcomere-like pattern was observed in the stress fibers of cultured bovine aortic endothelial cells recovered from cytochalasin D treatment.
Results of these studies suggest that : 1 The expression of endothelial stress fibers is induced by a cell elongation during embryonic development. 2 The endothelial stress fibers may support cell adhesion between cells or cell to substrate. 3 The sarcomere-like arrangement of actin microfilaments appeared in the developing stress fibers may represent one of the structural foundations.
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