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1998 Fiscal Year Final Research Report Summary

EXPRESSION OF Na^+-DEPENDENT GLUCOSE TRANSPORTERS (SGLT1, SGLT2) IN RAT KIDNEY PROXIMAL TUBULES.

Research Project

Project/Area Number 09670054
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionKITASATO UNIVERSITY

Principal Investigator

KAWAHARA Katsumasa  Kitasato Univ., School of Med.Professor, 医学部, 教授 (70134525)

Co-Investigator(Kenkyū-buntansha) YAJIMA Yoshitada  Kitasato Univ., School of Med.Prof., 医療衛生学部, 教授 (20050396)
ANZAI Naohiko  Kitasato Univ., School of Med.Res.Ass, 医学部, 助手 (70276054)
Project Period (FY) 1997 – 1998
KeywordsNa^+-dependent glucose transporter / Na^+-independent glucose transporter / glucocorticoid / neonatal rats / Diabetes Mellitus / renal proximal tubule
Research Abstract

In kidney proximal tubules, D-glucose is transported from the lumen to the blood via two-step processes : Na+-dependent/independent glucose transporters (SGLT1, 2/GLUTI, 2) at both apical and basolateral membranes, respectively. In kidney tubules of distal portions, only GLUT1 is expressed at the basolateral membrane to absorb D-glucose from the blood. Using Northern blot analysis we investigated the changes of the SGLT1, SGLT2, and GLUTi mRNA expressions in neonatal rat kidney tubules during early developmental period (4-15 days). A level of SGLTImRNA expression increased on the process of development : 100% (5 d), 330% (10 d), and '720% (15 d). Secondarily, a level of SGLT2 mRNA expression increased : 100% (5 d), 280% (10 d), and 42% (15 d). Finally, a level of GLUT1 mRNA expression increased : 100% (5 d), 118% (10 d), and 132% (15 d). Injection of betamethasone (60mug/100 g body wt. , i. p.) increased the level of SGLT1, SGLT2, and GLUTi mRNA expressions by 4-9 (5-day-old rats) and by 2-3 (15-day-old rats). These results indicate that the expression of Na+-dependent /independent glucose transporters mRNA increased during the developmental period of 4-15 days and strongly suggest that betamethasone is more effective in induction of the expression of the glucose transporters at earlier period of neonates.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Anzai, N.: "Roles of Vasopressin and Hypertonicity in Basolateral Na/K/2Cl Cotransporter Expression in Rat Kidney Inner Medullary Collecting Duct Cells." Jpn.J.Physiol.49(In Press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小久保麻子: "新生児ラット腎尿細管におけるNa^+依存性/非依存性グルコース輸送体遺伝子の発現" 発達腎研究会誌. 1(In Press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Anzai, N.: "Renal compensation for body water loss during dehydration in neonatal rats." Jpn.J.Physiol.48. 181-187 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori, M.: "Calcium release from intracellular stores evoked by extracellular ATP in a Xenopus renal epithelial cell line." J.Physiol(Lond). 502. 365-373 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 梅沢慎一: "電流知覚閾値(Current percetion threshold)による糖尿病性末梢神経障害の評価-新しい神経機能診断装置Neurometerを用いて-" 糖尿病. 40. 711-718 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawahara, K.: "Elsevier Science B.V." Molecular Machineries of the Neonatal Rat Kidney against Osmotic Stress., 8 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢島 義忠: "Modern Diabetes2 ; 治療-糖尿病性腎症" 糖尿病血管合併症の診断と治療, 10

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Anzai, N.: "Roles of vasopressin and hypertonicity in basolateral Na/K/2Cl cotransporter expreeion in rat kidney inner medullary collecting duct cells" Jpn.J.Physiol.49 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Anzai, N.: "Renal compensation for body water loss during dehydration in neonatal rats." Jpn.J.Physiol. 48. 181-187 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori, M: "Calcium release from intracellular stores evoked by extracellular ATP in a Xenopus renal epithelial cell line." J.Physiol (Lond). 502. 365-373 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawahara, K.: Molecular Machineries of the Neonatal Rat Kidney against Osmotic Stress.Elsevier Science B.V., 151-158 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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