• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1999 Fiscal Year Final Research Report Summary

Identification of novel oncogenes invloved in onset and progression of cancers with poor prognosis.

Research Project

Project/Area Number 09670145
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

UCHIDA Kazuhiko  Institute of Basic Medical Science, Department of Biochemistry and Molecular Oncology, University of Tsukuba Associate Professor, 基礎医学系, 助教授 (90211078)

Co-Investigator(Kenkyū-buntansha) TODOROKI Takeshi  Institute of Clinical Medicine, Department of Surgery, University of Tsukuba Associate Professor, 臨床医学系, 助教授 (70114105)
Project Period (FY) 1997 – 1999
Keywordsbiliary tract cancer / ovarian cancer / neuroblastoma / gastric cancer / DNA microarray / array CGH / prognosis / 1q23 amplification
Research Abstract

(1997) In spite of the development of clinical techniques, the prognoses of many cancers are still poor. Comparative genomic hybridization (CGH) is useful for identification of novel oncogenes and tumor suppressor genes involved in the carcinogenesis. 1) Biliary tract cancer ; CGH was performed on 30 fresh frozen tissues of gallbladder cancers. Chromosomal gain of 12p was associated with stage III and IV (P<0.05). Therefore, gain of 12p may be a potential prognostic factor of gallbladder cancers. 2) Ovarian cancer ; In order to find genetic changes in drug-resistant tumors, CGH was performed on 21 primary ovarian cancers and some cell lines. We found gains in chromosomal regions 1p, 1q and 19p, and losses in 2p and 15q to be related to the cisplatin-resistant phenotype. The cell lines which acquired the taxol-resistance had chromosomal gain of 7q where MDR gene was located. The amplification of MDR gene was confirmed by southern blot analysis. Present findings suggest that these chromo … More somal gains and losses may be potential indicators for prediction of resistance in ovarian cancer patients before cisplatin- and/or taxol-based chemotherapy. 3) Neuroblastoma ; We performed CGH on 24 neuroblastomas and dual-color FISH to identify genetic aberrations associated with progressive neuroblastoma. A novel chromosomal gain at 1q21-q25 was found in all of progressive stage 4 neuroblastomas. Furthermore, by FISH analysis using cosmid clones, the 1q21-q25 gain was narrowed to 1q23. These results suggest that DNA amplification at 1q23 may play a role in the development of progressive neuroblastoma in advanced stage.
(1998) To clarify the biological characteristics of regressive and progressive neuroblastomas, CGH was performed on 67 patients with neuroblastomas. The CGH data of all regressive tumors (stage1-3 and 4s) revealed whole-chromosome aberrations of whole portion of chromosome. On the other hand, progressive tumors revealed chromosomal gains and losses on regional portion of chromosome. Our data suggest that neuroblastomas are classified into two biologically different groups, one of which displays progressive disease which displays progressive disease which has partial chromosomal changes, whereas the other mimics advanced stage 3/4 neuroblastoma but displays regressive disease which has whole chromosomal aberrations.
(1999) Recent advances in DNA microarray allow us genome-wide analysis of genetic alterations including DNA copy number changes, mutations, and gene expression in cancers. We tried to establish array-based CGH with high resolutions, high quantitative capability and sensitivity, and applied this novel powerful method to mapping the putative oncogenes in cancers. Our arrayed CGH showed quantitative analysis of DNA copy number in the range from 1 to 10,000 copies. We detected 10 copies of the gene per cell. We also performed gene expression monitoring of gastric cancer using microarray with 4,000 cDNAs of known genes and identified many kinds of genes that were specifically expressed in gastric cancer. Less

  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Hirai M.et al.: "1q23 gain is associated with progressive neuroblastomas resistant toaggressive treatment"Genes。Chromosomes and Cancer. 28. 261-269 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 内田和彦: "ジーンチップの現状とポストゲノムにおける役割"生化学. 71. 119-124 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 内田和彦 他: "DNAマイクロアレイを用いたゲノムワイドの遺伝子増幅、欠失の解析"遺伝子医学. 4. 113-117 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 内田和彦、柏木浩暢: "マイクロアレイを用いたゲノム解析-遺伝子増幅と欠失"実験医学. 17. 1362-1366 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishikawa T.et al.: "Expression of α-fetoprotein and prostate-specific antigen genes in several tissues and detection of mRNAa in normal circulating blood by reverse transcriptase-polymerase chain reaction"Jpn. J. Clin. Oncol.. 28. 723-728 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hanai S.et al.: "Genomic organization of Drosophila poly(ADP-ribose) polymerase and distribution of its mRNA during development"J. Biol. Chem.. 273. 11881-11886 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirai, M., Yoshida, S., Kashiwagi, H., Kawamura, T., Ishikawa, T., Kaneko, M., Ohkawa, H., Nakagawa, A., Miwa, M., and Uchida, K.: "1q23 gain is associated with progressive neuroblastomas resistant to aggressive treatment."Genes, Chromosomes and Cancer.. 25. 261-269 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kudoh, K., Takano, M., Yoshida, S., Mano, Y., Yamamoto, K., Ishii, K., Kita, T., Kikuchi, Y., Nagata, I., Miwa, M., and Uchida, K.: "Gains of 1q21-q22 and 13q12-q14 are potential indicators for resistance to cisplatin-based chemotherapy in ovarian cancer patients."Clin. Cancer Res.. 5. 2526-2531 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miwa, M., Hanai, S., Poltronieri, P., Uchida, M., and Uchida, K.: "Functional analysis of poly(ADP-ribose) polymerase in Drosophila melanogaster."Mol. Cell. Biochem.. 193. 103-108 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koyama, Y., Katagiri, S., Hanai, S., Uchida, K., and Miwa, M.: "Poly(ADP-ribose) polymerase interacts with novel Drosophila ribosomal proteins, L22 and L23a, with unique histone-like amino-terminal extensions"Gene.. 226. 339-345 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Feng, R., Tanaka, M., Abe, H., Arashi, N., Sun, B., Uchida, K., and Miwa, M.: "Human T-cell leukemia virus type 1 can infect a wide variety of cells in mice."Jpn. J. Cancer Res.. 90. 48-54 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sun, B., Fang, J., Yagami, K., Kushida, S., Tanaka, M., Uchida, K., and Miwa, M.: "Age-dependent paraparesis in WKA rats : evaluation of MHC k-haplotype and HTLV-1 infection."Journal of Neurological Sciences.. 167. 16-21 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fang, J., Kushida, S., Feng, R., Tanaka, M., Kawamura, T., Abe, H., Maeda, N., Onobori, M., Hori, M., Uchida, K., and Miwa, M.: "Transmission of human T-cell leukemia virus type 1 to mice."J. Virol.. 72. 3952-3957 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maeda, N., Kawamura, T., Hoshino, H., Yamada, N., Blackard, J., Kushida, S., Miyano-Kurosaki, N., Yamamoto, N., Makino, K., Yokota, T., Uchida, K., and Miwa, M.: "Inhibition of human T-cell leukemia virus type 1 replication by antisense env oligodeoxynucleotide."Biochem. Biophys. Res. Commun.. 243. 109-112 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishikawa, T., Kashiwagi, H., Iwakami, Y., Hirai, M., Kawamura, T., Aiyoshi, Y., Yashiro, T., Ami, Y., Uchida, K., and Miwa, M.: "Expression of α-fetoprotein and prostate-specific antigen genes in several tissues and detection of mRNAs in normal circulating blood by reverse transcriptase-polymerase chain reaction."Jpn. J. Oncol.. 28. 723-728 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hanai, S., Uchida, M., Kobayashi, S., Miwa, M., and Uchida, K.: "Genomic organization of Drosophila poly(ADP-ribose) polymerase and distribution of its mRNA during development."J. Biol. Chem.. 273. 11881-11886 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamura, T., Hanai, S., Yokota, T., Hayashi, T., Poltronieri, P., Miwa, M., and Uchida, K.: "An alternative form of poly(ADP-ribose) polymerase in Drosophila melanogaster and its ectopic expression in Rat-1 cells."Biochem. Biophys. Res. Commun.. 251. 35-40 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] P. Poltronieri, T. Yokota, Y. Koyama, S. Hanai, K. Uchida and M. Miwa.: "PARP cleavage in the apoptotic pathway in S2 cells from Drosophila melanogaster."Biochemisitry and Cell Biology. 75. 445-449 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J. Fang, S. Kushida, R. Feng, M. Tanaka, H. Kikukawa, T. Kawamura, K. Uchida and M. Miwa.: "Integration of HTLV-1 provirus into mouse transforming growth factor-α gene."Biochem. Biophys. Res. Commun.. 233. 792-795 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2001-10-23  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi