1998 Fiscal Year Final Research Report Summary
Functional analysis of a novel human kinase, Aik, involved in chromosome segregation.
| Project/Area Number |
09670149
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Gifu University |
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Principal Investigator |
OKANO Yukio School of Medicine, Gifu University Professor, 医学部, 教授 (10177066)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Masashi School of Medicine, Gifu University Research Assoc., 医学部, 助手 (40260575)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Aik (Aurora / Ipll-related kinase) / centrosome / chromosome mapping / cell cycle / breast cancer |
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| Research Abstract |
It is known that mutants of fly Aurora and yeast IPL1 cause abnormal chromosome segregation resulting in infertility or aneuploidy. A novel protein kinase AIK1 (previously called Aik) shares high homology with Aurora and IPL1, and is expressed cell cycle-dependently at centrosome, suggesting that it may play an important role in chromosome segregation. AIK1 gene is mapped at human chromosome 20q13.2-3, which is known to be amplified in some cancer cells. Recent investigations revealed that AIK1 is an oncogene. In the present study, AIK1 expression was examined in tissues from patients with breast cancer. Irrespective of histological types, only cancer cells were stained in most of cases (31 out of 33), whereas benign tumors were stained weakly and in part (Manuscript submitted).
Database search indicated that AIK1 constitutes a protein kinase family, and we cloned the second and the third member, AIK2 and AIK3, respectively.
AIK2 cDNA encodes a 343 amino acid-protein, and AIK2 protein is highly expressed at M phase. AIK2 gene (STK12) was mapped at human chromosome 17p13.1, where p53 is known to localize closely and deletions are reported in some cancer cells. (Cytogenet. Cell Genet. 1998)
AIK3 cDNA encodes a 309 amino acid-protein, and AIK3 protein is highly expressed at centrosome during anaphase to cytokinesis. Some cancer cell lines exhibited higher expression of AIK3 than the others. AIK3 gene was mapped at human chromosome 19q13.43, where deletions are reported in some cancer cells. Thus, possible involvement of all 3 AIK family was suggested in tumorgenesis. (J.Biol. Chem. 1999)
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