1998 Fiscal Year Final Research Report Summary
Establishment of Immortalized Neuronal Cells from Transgenic Mice and Its Application to Neuronal Diseases
| Project/Area Number |
09670162
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Meijo University |
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Principal Investigator |
KANEDA Norio Meijo University, Faculty of Pharmacy, Professor, 薬学部, 教授 (00144139)
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| Co-Investigator(Kenkyū-buntansha) |
HIKITA Kiyomi Meijo University, Faculty of Pharmacy, Research Associate, 薬学部, 助手 (30257654)
YOKOYAMA Minesuke Mitsubishi Kasei Institute of Life Sciences, Cheaf, 生殖工学開発室, 室長
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| Project Period (FY) |
1997 – 1998
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| Keywords | Transgenic Mice / Substantia Nigra / Neurodegenerative Disease |
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| Research Abstract |
Parkinson's disease is a neurodegenerative disease in which a selective impairment of nigro-striatal dopaminergic neurons is occurred. To understand the basic mechanism of the selective cell death, nigro-striatal dopaminergic cell line would be extreamly useful. Since the cell number of dopaminergic neurons in substantia nigra is relatively low, and neurons don't proliferate, we used transgenic technology to overcome these problems.
In 1997, we constructed a transgene which is composed of 2.5 kb promoter region of human tyrosine hydroxylase (TH) gene fused to a coding region of SV40T.Using this DNA fragment, we produced 6 lines of Tg mice, in which 2 lines died about 8 weeks after birth and their offsprings were not obtained. The remaining 4 lines were maintained.
In 1998, we tried to culture mesencephalic neurons from the embryos of Tg mice. Cells were cultured at a permissive 33C for 1 week, and analysed by immunocytochemistry. In a double staining experiment, several immuno-positive clones for SV40T were found, but were not immuno-positive for TH.To make the isolation of neuronal cells more easy, we are planning to produce Tg mice carrying a neomycine registant gene under the same promoter region of TN.
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