1998 Fiscal Year Final Research Report Summary
Human hepatoma antigen analyzed by genetic engineering method
| Project/Area Number |
09670181
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | Gifu University School of Medicine |
|---|
Principal Investigator |
TAKAMI Tsuyoshi Gifu University School of Medicine, Professor, 医学部, 教授 (70136943)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Takashi Gifu University School of Medicine, Assistant, 医学部, 助手 (20293558)
SAIO Masanao Gifu University School of Medicine, Assistant, 医学部, 助手 (40242721)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Keywords | Hepatoma / HLA-A24 / genetic engineering / Hpe.3B2.1-7 / Acid elution / FPLC / Peptide |
|---|
| Research Abstract |
Human hepatoma cell line, Hep.3B2.1-7 that has no HLA-A24 gene expression, has been transduced of HLA-A24 cDNA by using mammalian cell line expression vector pcDNA3 .1 in order to carry the endogenous antigenic peptides on its cell surface. The acidic eluate of cloned HLA-A24 expressing Hep.3B2.1-7 (Hep.3B.A24) demonstrated seven significant peakes that were different from wild type Hep.3B2.1-7 and moke-transfected Hep.3B2.1-7 after FPLO fractionations.
One out of seven peptide fraction had anchoring motief for HIA-A24 at second (L) and tenth (K) positions, while two histon H2A, one HLA-A68 binding peptide of macrophage migration inhibiting factor, one HIA-B35 binding peptide, and two unknown peptides were clarified from remaining six fractions.
The homology research of HLA-A24 binding peptide obtained in present study demonstrated no homologous protein in data base.
In conclusion, present study is possible to clarify a novel HLA-A24 binding peptide, and HLA.molecules enforced to express by genetic engineering method will open a new avenue for the research in tumor-antigenic peptides.
|
