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1998 Fiscal Year Final Research Report Summary

Role of cyclin E as an oncogene for human gastrointestinal tract cancer

Research Project

Project/Area Number 09670186
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

YOKOZAKI Hiroshi  Hiroshima University Faculty of Medicine, Assistant Professor, 医学部, 講師 (10200891)

Project Period (FY) 1997 – 1998
KeywordsHuman / Digestive system / Gastric cancer / Colorectal cancer / Cyclin E / Transfection / Antisense vector / Oncogene
Research Abstract

1. Construction of cyclin E expression vector and introduction to mouse NIH/3T3 fibroblast
(1) A 2.5 Kbp fragment of cyclin E cDNA that contained its total open reading frame was subcloned in to EcoRI site of mammalian expression vector pCDNA3 with cytomegalovirus promotor.
(2) The cyclin E expression vector or mock pCDN3 were transfected to mouse NTH/3T3 fibroblast by lipofection method. After G418 selection, each 10 clones were recovered. Integration of the vectors and expression of recombinant cyclin E protein were confirmed by Southern and Western blot analysis, respectively.
(3) Significant difference in growth rates were not observed between cyclin E transvectant and mock transfectant. No transformation focus was detected in cyclin E transfectants.
2. Construction of cyclin E antisense vector and introduction to human gastric cancer cell lines.
(1) A 11theta bp cyclin E cONA fragment was subcloned in antisense direction to pCDNA3 vector.
(2) antisense cyclin E vector was transfected into human gastric cancer cell lines (TMK.-1, MKN-7, MKN-28 and MKN-74). Each 10 G418 resistant clones were recovered.
(3) Significant difference in growth rates were not observed between antisense cyclin E transfectant and mock transfectant.
These in vitro observations in the present research project provided any direct evidence supporting the idea that cyclin E can act as oncogene in human gastrointestinal carcinogenesis.

  • Research Products

    (78 results)

All Other

All Publications (78 results)

  • [Publications] Yasui W: "Expression of p21 WAF1/CIP1 in colorectal adenomas and adenocarcinomas and its correlation with p53 protein expression" Pathol Int. 47・7. 470-477 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasui W: "Reduced expression of cyclin-dependent kinase inhibitor p27Kip1 is associated with advanced stage and invasiveness of gastric carcinomas" Jpn J Cancer Res. 88・7. 625-629 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuniyasu H: "Growth-inhibitory effect of interferon-beta is associated with the induction of cyclin-denepdent kinase inhibitor,p27KIP1 in a human gastric carcinoma cell line" Cell Growth & Differ. 8・1. 47-52 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuniyasu H: "Expression of human telomerase RNA is an early event of stomach carcinogenesis" Jpn J Cancer Res. 88・2. 103-107 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kannan S: "Telomerase activity in premalignant and malignant lesions of human oral mucosa" Cancer Epidemiol Biomarkers Prev. 6・6. 413-420 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naka K: "Growth inhibition of cultured human gastric cancer cells by 9-cis-retinoic acid with induction of cdk inhibitor Waf1/Cip1/Sdi1/p21 protein" Differentiation. 61・5. 313-320 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hamamoto T: "Altered microsatellites in incomplete-type intestinal metaplasia adjacent to primary gastric cancers" J Clin Pathol. 50・10. 841-846 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakahara M: "Identification of concurrent germ-line mutations in hMSH2 and/or hMLH1 in Japanese hereditary nonpolyposis colorectal cancer kindreds" Cancer Epidemiol Biomarkers Prev. 6・12. 1057-1064 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kudo Y: "Overexpression of CDK-activating CDC25B phosphatase in human gastric carcinomas" Jpn J Cancer Res. 88・10. 947-952 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito R: "Growth stimulatory effect of interleukin(IL)-1α produced by oral squamous cell carcinoma cell lines" Int J Oncol. 807-812 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito R: "Expression of IL-6 and its effect on the cell growth of gastric carcinoma cell lines" Jpn J Cancer Res. 88・10. 953-958 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasui W: "Expression of CD44 containing variant exon9(CD44v9) in gastric adenomas and adenocarcinomas:Relation to the proliferatiion and progression" Int J Oncol. 12・6. 1253-1258 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Semba S: "Frequent microsatellite instability and loss of heterozygosity in the region including BRCA1(17q21) in young patients with gastric cancer" Int J Oncol. 12・6. 1245-1251 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto S: "Expression of vascular endothelial growth factor in human gastric carcinomas" Pathol Int. 48・7. 499-506 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiyama T: "Frequent p53 gene mutations in serrated adenomas of the colorectum" J Pathol. 186・2. 131-139 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiyama T: "In situ mRNA hybridization technique for analysis of human telomerase RNA in gastric precancerous and cancerous lesions" Jpn J Cancer Res. 89・11. 1187-1194 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ue T: "Co-expression of osteopontin and CD44v9 in gastric cancer" Int J Cancer. 79・2. 127-132 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ue T: "Reduced expression of the CD44 variant exons in oral squamous cell carcinoma and its relationship to metastasis" J Oral Pathol Med. 27・5. 197-201 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kiriu H: "Microsatellite instability associated with primary head and neck cancers and secondary esophageal cancers" Jpn J Clin Oncol. 28・12. 733-739 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shitara Y: "Mutation of the transforming growth factor-β type II receptor gene is rare event in human gastric carcinomas" Int J Oncol. 12・5. 1061-1065 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitadai Y: "Expression of interleukin-8 correlates with vascularity in human gastric carcinomas" Am J Pathol. 152・1. 93-100 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokozaki H: "Allele frequency of D17S855 microsatellite locus in Japanese people" Hum Hered. 49・1. 61-62 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokozaki H: "Microsatellite instabilities in gastric cancer patients with multiple primary cancers" Int J Oncol. 14・1. 151-155 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shitara Y: "No mutation of the Smad2 gene in human sporadic gastric carcinomas" Jpn J Clin Oncol. 29・1. 3-7 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokozaki H: "Allele frequency of D1S191 microsatellite locus in Japanese people" J Exp Clin Cancer Res. 印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokozaki H: "Effect of 3,4-dihydro-6-[4-(3,4-dimethoxybenzoyl)-1-piperazinyl]-2(1H)-quinolinone (vesnarinone) on the growth of gastric cancer cell lines" Cancer Lett. 印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiyama T: "Overexpression of telomerase RNA is an early event of esophageal carcinogenesis" Virchows Arch. 印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naka K: "Effect of antisense human telomerase RNA(hTR) transfection on the growth of human gastric cancer cell lines" Biochem Biophys Res Commun. 印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki T: "Expression of the E2F family in human gastrointestinal carcinomas" Int J Cancer. 印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokozaki H: "Molecular Pathology of Gastroenterological Cancer-Application to Clinical Practice.Tahara E(ed)" Springer-Verlag, 267 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tahara E: "Progress in Gastric Cancer Research 1997,Ruediger Siewert J and Roder JD(eds)" Monduzzi Editore, 1794 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 横崎 宏: "入村達郎・編、続=がんの浸潤・転移マニュアル" 金芳堂, 267 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 横崎 宏: "〓香 正博・編、最新内科学体系 プログレスシリーズ 消化器疾患" 中山書店, 383 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田原榮一: "黒川 清、他・編、EBM現代内科学" 金芳堂, 2384 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 横崎 宏: "藤盛孝博・編、大腸の臨床分子病理学 形態と遺伝子診断" メジカルビュー社, 211 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 横崎 宏: "横田 淳・編、イラスト医学&サイエンスシリーズ 癌化のメカニズムを解く" 羊土社, 124 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tahara E: "7th International Cancer Congress,Morares M,Brentani R and Bevilacqua R(eds)" Monduzzi Editore, 1673 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tahara E: "Gastritis,Graham DY,Genta RM and Dixon MF(eds)" Lippincott Williams and Wilkins印刷中, (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tahara E: "Molecular Pathology of Early Cancer,Srivastava S,Henson DE and Gazdar A(eds)" IOS Press印刷中, (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasui W: "Expression of p21^<WAFI/CIPI> in colorectal adenomas and adenocarcinomas and its correlation with p53 protein expression" Pathol Int. 47-7. 470-477 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasui W: "Reduced expression of cyclin-dependent kinase inhibitor p27^<Kipl> is associated with advanced stage and invasiveness of gastric carcinomas" Jpn J Cancer Res. 88-7. 625-629 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuniyasu H: "Growth-inhibitory effect of interferon-beta is associated with the induction of cyclin-denepdent kinase inhibitor, p27^<KIPI> in a human gastric carcinoma cell line" Cell Growth & Differ. 8-1. 47-52 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuniyasu H: "Expression of human telomerase RNA is an early event of stomach carcinogenesis" Jpn J Cancer Res. 88-2. 103-107 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kannan S: "Telomerase activity in premalignant and malignant lesions of human oral mucosa" Cancer Epidemiol Biomarkers Prev. 6-6. 413-420 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naka K: "Growth inhibition of cultured human gastric cancer cells by 9-cis-retinoic acid with induction of cdk inhibitor Waf1/Cip1/Sdi1/p21 protein" Differentiation. 61-5. 313-320 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hamamoto T: "Altered microsatellites in incomplete-type intestinal metaplasia adjacent to primary gastric cancers" J Clin Pathol. 50-10. 841-846 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakahara M: "Identification of concurrent germ-line mutations in hMSH2 and/or hMLH1 in Japanese hereditary nonpolyposis colorectal cancer kindreds" Cancer Epidemiol Biomarkers Prev. 6-12. 1057-1064 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kudo Y: "Overexpression of CDK-activating CDC25B phoshatase in human gastric carcinomas" Jpn J Cancer Res. 88-10. 947-952 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito R: "Growth stimulatory effect of interleukin(IL)-1alpha produced by oral squamous cell carcinoma cell lines" Int J Oncol. 11. 807-812 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito R: "Expression of IL-6 and its effect on the cell growth of gastric carcinoma cell lines" Jpn J Cancer Res. 88-10. 953-958 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasui W: "Expression of CD44 containing variant exon 9(CD44v9)in gastrric adenomas and adenocarcinomas : Relation to the proliferation and progression" Int J Oncol. 12-6. 1253-1258 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Semba S: "Frequent microsatellite instability and loss of heterozygosity in the region including BRCAl(17q21)in young patients with gastric cancer" Int J Oncol. 12-6. 1245-1251 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto S: "Expression of vascular endothelial growth factor in human gastric carcinomas" Pathol Int. 48-7. 499-506 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiyama T: "Frequent p53 gene mutations in serrated adenomas of the colorectum" J Pathol. 186-2. 131-139 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiyama T: "In situ mRNA hybridization technique for analysis of human telomerase RNA in gastric precancerous and cancerous lesions" Jpn J Cancer Res. 89-11. 1187-1194 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ue T: "Co-expression of osteopontin and CD44v9 in gastric cancer" Int J Cancer. 79-2. 127-132 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ue T: "Reduced expression of the CD44 variant exons in oral squamous cell carcinoma and its relationship to metastasis" J Oral Pathol Med. 27-5. 197-201 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiriu H: "Microsatellite instability associated with primary head and neck cancers and secondary esophageal cancers" Jpn J Clin Oncol. 28-12. 733-739 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shitara Y: "Mutation of the transforming growth factor-beta type II receptor gene is rare event in human gastric carcinomas" Int J Oncol. 12-5. 1061-1065 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitadai Y: "Expression of interleukin-8 correlates with vascularity in human gastric carcinomas" Am J Pathol. 152-1. 93-100 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokozaki H: "Allele frequency of D17S855 microsatellite locus in Japanese people" Human Hered. 49-1. 61-62 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokozaki H: "Microsatellite instabilities in gastric cancer patients with multiple primary cancers" Int J Oncol. 14-1. 151-155 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shitara Y: "No mutation of the Smad2 gene in human sporadic gastric carcinomas" Jpn J Clin Oncol. 29-1. 3-7 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokozaki H: "Allele frequency of D1S191 microsatellite locus in Japanese people" J Exp Clin Cancer Res. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokozaki H: "Effect of 3,4-dihydro-6-[4-(3,4-dimethoxybenzoyl)-1-piperazinyl]-2(1H)-quinolinone(vesnarinone)on the growth of gastric cancer cell lines" Cancer Lett. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiyama T: "Overexpression of telomerase RNA is an early event of esophageal carcinogenesis" Virchows Arch. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naka K: "Effect of antisense human telomerase RNA(hTR)transfection on the growth of human gastric cancer cell lines" Biochem Biophys Res Commun. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki T: "Expression of the E2F family in human gastrointestinal carcinomas" Int J Cancer. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokozaki H: Molecular Pathology of Gastroenterological Cancer-Application to Clinical Practice-.Tahara E(ed). Springer-Verlag, 267 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tahara E: Progress in Gastric Cancer Research 1997, Ruediger Siewert J and Roder JD(eds). Monduzzi Editore, 1794 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokozaki H: Manual for Invasion and Metastasis Research(2nd Series), Irimura T(ed)(in Japanese). Kinpodo, 267 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokozaki H: Integrated Handbook of Internal Medicine, Diseases of the Digestive System, Asaka M(ed)(in Japanese). Nakayama Syoten, 383 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tahara E: EBM Textbook of Internal Medicine, Kurokawa K et al(eds)(in Japanese). Kinpodo, 2384 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokozaki H: Clinical Molecular Pathology of Colon, Fujimori T(ed)(in Japanese). Midicao View Co., 211

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokozaki H: Mechanisms of Carcinogenesis, Yokota J(ed)(in Japanese). Yodo-Sya, 124

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tahara E: 17th International Cancer Congress, Morares M,Brentani R and Bevilacqua R(eds). Monduzzi Editore, 1673 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tahara E: Gastritis, Graham Dy, Genta RM and Dixon MF(eds). Lippincott Williams and Wilkins, (in press), 1999

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tahara E: Srivastava S,Henson DE and Gazdar A(eds). IOS Press, (in press), 1999

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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