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1998 Fiscal Year Final Research Report Summary

Pathological Analysis of Expression of Extracellular Matrix in Glomerulonephritis

Research Project

Project/Area Number 09670192
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionNagasaki University

Principal Investigator

RAZZAQUE Mohammed Shawkat  Nagasaki University School of Medicine, Assistant, 医学部, 助手 (50264213)

Project Period (FY) 1997 – 1998
KeywordsGlomerulonephritis / collagen / heat shock protein 47 / fibrosis / glomerulosclerosis / Pulmonary fibrosis / Extracellular matrix / Renal biopsy
Research Abstract

To explore the possible role of heat shock protein (HSP) 47 in the process of sclerosis/fibrosis of renal and pulmonary tissue, molecular pathological analysis was performed using experimental animal models and human tissues.
In the experiments of senile model (Fischer 344 rat) and gentamicin-induced renal injury, the expression of HSP was increased in sclerotic glomeruli and fibrotic interstitium. Double immunostaining revealed co-expression of HSP47 and alpha-smooth muscle actin/vimentin. It is suggested that HSP47 may be produced by phenotypically changed cells in these models. In human renal biopsy tissue of diabetic nephropathy and IgA nephropathy, accumulation of collagen type III and collagen type IV was associated with increased expression of HSP47. This is the first study concerning the expression of HSP47 in human renal diseases. Using renal biopsy specimens of human mesangial proliferative glomerulonephritis, collagen type VI was shown to present in the glomerulus, and to increase in amount in proliferative and sclerotic regions. in situ hybridization revealed glomerular epithelial cells may produce collagen type VI in the conditions. By immunohistochemical study of human fibrotic lung diseases, colocalization of collagen and HSP47 was noted in fibrotic lesions.
From these results, it is concluded that overexpression of HSP47 may play an important role in the excessive assembly/synthesis of collagens and can thereby contribute to sclerosis/fibrosis in chronic glomerular disease and pulmonary fibrotic diseases.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] M.S.Razzaque,T.Taguchi et al.: "Age-related nephropathy in the Fischer 344 rat is associated with overexpression of collagens and collagen-binding heat shock protein 47" Cell Tissue Res. 293. 471-478 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.S.Razzaque,T.Taguchi et al.: "Immunolocalization of Collagen and Collagen-Binding Heat Shock Protein 47 in Fibrotic Lung Diseases" Mod Pathol. 11(12). 1183-1188 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.S.Razzaque,T.Taguchi et al.: "Coexpression of Collagenand Collagen-Binding Heat Shock Protein 47 in Human Diabetic Nephropathy and IgA Naphropathy" Nephron. 80. 434-443 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.S.Razzaque,T.Taguchi et al.: "Expression of the heat shock protein 47 in gentamicin-treated rat kidneys" Int.J.Exp.path.79. 125-132 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.S.Razzaque,T.Taguchi et al.: "Localization in situ of type VI collagen protein and its mRNA in mesangial proliferative glomerulonephritis using renal biopsy sections" Histochem Cell Biol. 111. 1-6 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.S.Razzaque,T.Taguchi et al.: "Life-long dietary restriction modulates the expression of collagens and collagen-binding heat shock proitein 47 in aged Fischer 344 rat kidney" Histochem.J.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.S.Razzaque,T.Taguchi et al.: "Cisplatin-induced apoptosis human proximal tubular epithelial cells is associated with the activation of Fas/Fas ligand system" Histochem Cell Biol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.S.Razzaque,T.Taguchi et al.: "Possible role of glomerular epithelial cell-derived HSP 47 in carly glomerular injury in rats with dietary induced hypercholesterolemia" Kidney Intern. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.S.Razzaque,T.Taguchi et al.: "Localization of HSP 47 in cisplatin-treated kidny : a possible role in tubulinterstitial damage" Clin.Exper.Nephrol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.S.Razzaque, I.Shimokawa, A.Nazneen, Y.Higami, T.Taguchi: "Age-related nephropathy in the Fischer 344 rat is associated with overexpression of collagens and collagen-binding heat shock protein 47." Cell Tissue Res. 293. 471-478 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] MS.Razzaque, A.Nazneen, T.Taguchi: "Immunolocalization of Collagen and collagen-binding heat shock protein 47 in fibrotic lung disease." Modern Pathology. 11 (12). 1183-1188 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.S.Razzaque, A.Kumatori, T.Harada, T.Taguchi: "Coexepression of Collagens and Collagen-Binding Heat Shock Protein 47 in Human Diabetic Nephropathy and IgA Nephropathy." Nephron. 80. 434-443 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Cheng, M.S.Razzaque, A.Nazneen, T.Taguchi: "Expression of the heat shock protein 47 in gentamicin-traeted rat kidneys." Int.J.Exp.Path.79. 125-132 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] MS.Razzaque, T, Koji, T.Harada, T.Taguchi: "Localization in situ of type VI collagen protein and its mRNA in mesangial proliferative glomerulonephritis using renal biopsy sections." Histochem.Cell Biol.111. 1-6 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.S.Razzaque, I.Shimokawa, A.Nazneen, D.Liu, T.Naito, K.Higami, T.Taguchi: "Life-long dietary restriction modulates the expression of collagens and collagen-binding heat shock protein 47 in aged Fischer 344 rat kidney." Histochem.J.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.S.Razzaque, T.Taguchi, et al: "Cisplatin-induced apoptosis in human proximal tubular epithelial cells is associated with the activation of Fas/Fas ligand system." Histochem Cell Biol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.S.Razzaque, T.Taguchi: "Possible role of glomerular epithelial cell-derived HSP 47 in early glomerular injury in rats with dietary induced hypercholesterolemia." Kindney Intern. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.S.Razzaque, T.Taguchi: "Localization of HSP 47 in cisplatin-treated kidney : a possible role in tubulointerstitial damage." Clin.Exper.Nephrol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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