1998 Fiscal Year Final Research Report Summary
The role of telomerase activity in thyroid tumorigenesis
| Project/Area Number |
09670202
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | KITASATO UNIVERSITY |
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Principal Investigator |
OKAYASU Isao Kitasato Univ., School of Medicene, Professor, 医学部, 教授 (20014342)
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| Co-Investigator(Kenkyū-buntansha) |
MIKAMI Tetuo Kitasato Univ., School of Medicene, Research Associate, 医学部, 助手 (90286352)
SAEGUSA Makoto Kitasato Univ., School of Medicene Assistant Professor, 医学部, 講師 (00265711)
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| Project Period (FY) |
1997 – 1998
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| Keywords | thyroiditis / thyroid tumors / telemerase / telemere length / differentiation / cell proliferation / apoptosis |
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| Research Abstract |
Telomerase activity was examined in thyroid disease states by telomeric repeat amplification protocol assay, including adenomas arid carcinomas, and correlated with Clinicopathological features. Of a total of 26 papillary carcinomas, 16 cases (61.5%) were positive, with the poorly differentiated subtype being predominant (P<O.O5). A significantly more shortened terminal restriction fragment length (P<O.05), higher incidence of extrathyroidal extension (P<O.OO1), and more elevated Ki-67 labeling indices (P<O.002) were also found in telomerase-positive than in -negative papillary carcnnomas. Of four follicular carcinomas, 3 cases (75.5%) were positive. Positive telomerase activity in follicular adenomas (9/23 cases, 39.1%) and lymphocytic thyroiditis (12/22 cases, 54.5%) appeared to be mainly caused by infiltrating lymphocytes. However, three cases of atypical adenoma with relatively increased Ki-67 labeling indices were positive, suggesting a possibility of malignant potential. The good correlation with extrathyroidal invasiveness, Ki-67 labeling indices and poor differentiation of papillary carcinomas which was determined by the use of multivariate analysis suggests that this parameter might have potential application in estimation of tumor progession and prognosis and in clinical management.
Similarly, the relation of telomerase activity with cancer cell differentiation, proliferation and progression was confirmed in sporadic colorectal carcinomas.
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