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1998 Fiscal Year Final Research Report Summary

Intracellular localization of multidrug resistance associated protein (MRP) and atypical multidrug resistance phenomenon in non-small cell lung cancer

Research Project

Project/Area Number 09670205
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionTokai University

Principal Investigator

NAKAMURA Masato  Tokai University School of Medicine Assistant Professorr, 医学部, 講師 (00164335)

Co-Investigator(Kenkyū-buntansha) KIJIMA Hiroshi  Tokai University School of Medicine Assistant Professor, 医学部, 講師 (90204859)
YAMAZAKI Hitoshi  Tokai University School of Medicine Assistant Professor, 医学部, 講師 (20191273)
Project Period (FY) 1997 – 1998
Keywordslung cancer / multidrug resistance / MRP gene / molecular pathology
Research Abstract

Multidrug resistance-associated protein (MRP) is one of the major factors for non-P-glycoprotein (PGp)-mediated multidrug-resistance. It is unclear how MRP expression is regulated in NSCLC.In this study, we examined MRP and mutant p53 expression in 107 NSCLC by immunohistochemical procedures. Forty-seven of these 107 NSCLC cases were positive for MRP in the cytoplasm. Mutant p53-positive NSCLC showed a significant correlation with MRP overexpression. Coexpression of MRP and p53 in the same cells of NSCLC was confirmed by double staining procedures. Twenty-six patients with MRP-positive tumors who underwent postoperative chemotherapy with MRP-related anticancer drugs showed significantly poorer prognosis than those with MRP-negative tumors. This correlation between MRP expression and prognosis was also seen in stage Ill patients, and in patients with squamous cell carcinoma. NSCLC patients with coexpression of MRP and p53 showed poorer prognosis than those without MRP and p53. These results suggest that MRP overexpression affected by mutant p53 has a significant effect on prognosis through atypical non-PGp-mediated multidrug resistance in NSCLC.

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Nakamura Masato: "Gene Expression of Granulocyte Colony Stimulating Factor (G-CSF) in Non-Small Cell Lung Cancer" Anticancer Research. 17. 573-576 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Abe Yoshiyuki: "A Xenograft Line of Human Teratocarcinoma Established by Serial Transplantation in Sever Combined Immunodeficient (SCID) Mice" Acta Pathologica, Microbiologica et Immunologica Scandinavica(APMIS). 105. 283-289 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takamiya Yukihito: "Murine P-Glycoprotein on Stromal Vessels Mediates Multidrug Resistance in Intracerebral Human Glioma Xenografts" British Journal of Cancer. 76. 445-450 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsugu Atsushi: "Localization of Aberrant Messenger RNA of Epidermal Growth Factor Receptor (EGFR) in Malignant Glioma" Anticancer Research. 17. 2225-2232 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suto Ryuji: "A Case of Malignant Schwannoma with Overexpression of Multidrug Resistance Gene (MDR1) after Chemotherapy" Anticancer Research. 17. 2273-2278 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suto Ryuji: "Multidrug Resistance Mediated by Overexpression of P-Glycoprotein in Human Osteosarcoma in Vivo" International Journal of Oncology. 12. 287-291 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamazaki Hitoshi: "Inhibition of tumor growth by ribozyme-mediated suppression of aberrant epidermal growth factor receptor gene expression." Journal of National Cancer Institute. 90. 581-587 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oshika Yoshiro: "A human lung cancer xenograft producing granulocyte-colony stimulating factor and parathyroid hormone-related protein." Oncology Report. 5. 359-362 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokunaga Tetsuji: "Vascular endothelial growth factor (VFGF) mRNA isoform expression pattern is correlated with liver metastasis and poor prognosis in colon cancer." British Journal of Cancer. 77. 998-1002 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokunaga Tetsuji: "Is xenotransplantability of human colon cancers in SCID mice affected by angiogenic factors?" Journal of National Cancer Institute. 90. 400-401 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oshika Yoshiro: "Multidrug resistance-associated protein (MRP) and mutant p53 protein expression in non-small cell lung cancer." Modern Pathology. 11(11). 1059-1063 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukushima Yoshitaka: "Multidrug resistance-associated protein (MRP) expression is correlated with aberration of p53 protein in colorectal cancer." European Journal of Cancer. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masato Nakamura, Yoshiro Oshika, Yoshiyuki Abe, Yuichi Ozeki, Yuko Katho, Hitoshi Yamazaki, Hiroshi Kijima, Yoshito Ueyama, Toshiro Ogata, Norikazu Tamaoki: ""Gene expression of granulocyte colony-stimulating factor (G-CSF) in non-small cell lung cancer"" Anticancer Res. 17. 573-576 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiyuki Abe, Yoshiro Oshika, Yasuyuki Ohnishi, Ryuji Suto, Tetsuji Tokunaga, Hitoshi Yamazaki, Hiroshi Kijima, Nobuyoshi Hiraoka, Yoshito Ueyama, Norikazu Tamaoki, Masato Nakamura: ""A xenograft line of human teratocarcinoma established by serial transplantation in severe combined immunodeficient (SCID) mice"" APMIS. 105. 283-289 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yukihito Takamiya, Yoshiyuki Abe, Atsushi Tsugu, Yoshiro Oshika, Yasuyuki Ohnishi, Osamu Sato, Hitoshi Yamazaki, Hiroshi Kijima, Norikazu Tamaoki, Masato Nakamura: ""Murine glycoprotein on stromal vessels mediates multidrug resistance in intracerebral human glioma xenografts"" Br J Cancer. 76. 445-450 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Atsushi Tsugu, Hiroshi Kijima, Hitoshi Yamazaki, Yasuyuki Ohnishi, Yukihito Takamiya, Yoshiyuki Abe, Yoshito Ueyama, Osamu Sato, Norikazu Tamaoki, MasatoNakamura: ""Localization of aberrant messenger RNA of epidermal growth factor receptor (EGFR) in malignant glioma"" Anticancer Research. 17. 2225-2232 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ryuji Suto, Yoshiyuki Abe, Yoon Hwan Lee, Yoshito Ueyama, Hitoshi Yamazaki, Hiroshi Kijima, Nobuyoshi Hiraoka, Hiroaki Fukuda, Norikazu Tamaoki, Masato Nakamura: ""A case of malignant schwannoma with overexpression of multidrug resistance gene (MDR1) after chemotherapy"" Anticancer Research. 17. 2273-2278 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ryuji Suto, Yoshiyuki Abe, MasatoNakamura, Yasuyuki Onishi, Masumi Yoshimura, Yoon Hwan Lee, Takashi Imanishi, Hitoshi Yamazaki, Hiroshi Kijima, Testuji Tokunaga, Yoshiro Oshika, Nobuyoshi Hiraoka, Norikazu Tamaoki, Hiroaki Fukuda, Yoshito Ueyama: ""Multidrug resistance mediated by overexpression of P-glycoprotein in human osteosarcoma in vivo"" Intl J Onc. 12. 287-291 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hitoshi Yamazaki, Hiroshi Kijima, Yasuyuki Ohnishi Y,Yoshiyuki Abe, Yoshiro Oshika, Takashi Tsuchida, Tetsuji Tokunaga, Atsush Tsugu, Yoshito Ueyama Norikazu Tamaoki, Masato Nakamura: "Inhibition of tumor growth by ribozyme-mediated suppression of aberrant epidermal growthfactor receptor gene expression." J Natl Cancer Inst. 90. 581-587 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiro Oshika, Masato Nakamura M,Hiroyuki Hatanaka, Yoshiyuki Abe, Tetsuji Tokunaga, Yasuyuki Ohnishi, Hiroshi Kijima, Hitoshi Yamazaki, Narikazu Tamaoki, Yoshito Ueyama: "A human lung cancer xenograft producing granulocyte-colony stimulatingfactor and parathyroid hormone-relatedprotein." Oncol Rep. 5. 359-362 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tetsuji Tokunaga, Yoshiro Oshika, Yoshiyuki Abe, Yuichi Ozeki, Sotaro Sadahiro, Hiroshi Kijima, Takashi Tsuchida, Hitoshi Yamazaki, Yoshito Ueyama, Norikazu Tamaoki, Masato Nakamura: "Vascular endothelial growth factor (VFGF) mRNA isoform expression pattern is correlated with liver metastasisand poor prognosis in colon cancer." BrJ Cancer. 77. 998-1002 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tetsuji Tokunaga, Masato Nakamura, Yoshiro Oshika, Yasuyuki Ohnishi, Yoshito Ueyama.: "Is xenotransplantability of human colon cancers in SCID mice affected by angiogenic factors?" J Natl Cancer Inst.90. 400-401 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiro Oshika, Masato Nakamura, Tetsuji Tokunaga, Yoshitaka Fukushima, Yoshiyuki Abe, Yuichi Ozeki, Hitoshi Yamazaki, Norikazu Tamaoki, Yoshito Ueyama.: "Multidrug resistance-associatedprotein (MRP) and mutant p53 protein expression in non-small cell lung cancer." Mod Pathol. 11 (11). 1059-1063 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshitaka Fukushima, Yoshiro Oshika, Tetsuji Tokunaga, Hiroyuki Hatanaka, Masashi Tomisawa, Kenji Kawai, Yuichi Ozeki, Takashi Tsuchida, Hiroshi Kijima, Hitoshi Yamazaki, Yoshito Ueyama, Norikazu Tamaoki, Masato Nakamura.: "Multidrug resistance-associatedprotein (MRP) expression is correlated with aberration of p53 protein in colorectal cancer." Eur J Cancer. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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