1998 Fiscal Year Final Research Report Summary
Molecular Genetic Analysis of Synovial Sarcoma and its Application for Histologic Diagnosis
| Project/Area Number |
09670209
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
NOJIMA Takayuki Kanazawa Medical University, School of Medicine Professor, 医学部, 教授 (50142732)
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| Co-Investigator(Kenkyū-buntansha) |
NAGASHIMA Kazuo Hokkaido University, School of Medicine Professor, 医学部, 教授 (50010377)
TAKEGAMI Tsutomu Kanazawa Medical University, Medical Research Institute Assocoate Professor, 総合医学研究所, 助教授 (10113490)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Synovial sarcoma / Malignant soft tissue tumors / Gene rearrangements / SYT gene / SSX gene / Histologic diagnosis |
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| Research Abstract |
We studied 92 malignant bone and soft tissue tumors on the histological examination and molecular gene expression.
1) Detection of chimeric transcripts by RT-PCR
Total RNA was extracted from frozen specimens. RNA was reverse transcribed and the resulting cDNA was amplified by PCR using specific primers for detection of the chimeric transcripts. 15 tumors showed a major 585-bp DNA fragment of SYT-SSX.14 ; EWS-Flil, 8 ; PAX-FKHR, 4 ; EWS-ATF1.10 of 15 tumors had SYT-SSX chimeric transcript were initially diagnosed as synovial sarcoma. Other 5 tumors were initially unclassified or were considered synovial sarcoma in unusual locations ; bone, pericardium, retroperitoneum. The SSX1 gene was involved in 11, and the SSX2 gene ; 4 tumors.
2) Detection of normal SSX gene expression
Although normal SSX gene was only expressed in normal testis and thyroid gland, expression of SSX gene using by in situ hybridization was found in the epithelial component, fibrous areas and poorly differentiated cells of synovial sarcoma cases. Northern blot analysis with SSX1 probe detected rearranged SSX1 in all of synovial sarcoma cases, 6 Ewing's sarcoma, one embryonal rhabdomyosarcoma.
3) Application for histological diagnosis
RT-PCR can be considered a useful molecular biological technique that can provide objective evidence for differential diagnosis between synovial sarcoma and other malignant bone and soft tissue tumors ; Ewing's sarcoma, alveolar rhabdomyosarcoma, myxoid liposarcoma, claer cell sarcoma.
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