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1998 Fiscal Year Final Research Report Summary

Novel genes expressed during megakaryocytic differentiation

Research Project

Project/Area Number 09670245
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

FUSE Akira  Laboratory of Physicochemistry and Department of Safety, Research on Biologics, National Institution of Infectious Diseases, Chief, 安全性研究部, 室長 (60110300)

Co-Investigator(Kenkyū-buntansha) SATO Takeyuki  Department of Pediatrics, School of Medicine, Chiba University, Associate Proffe, 医学部, 助教授 (30187207)
Project Period (FY) 1997 – 1998
KeywordsMegakaryocyte differentiation / Erythrocyte differentiation / platelet production, enucleation / 脱核 / 巨核化 / 細胞構造変化
Research Abstract

1. Establishment of Megakaryoblastic cell line
A novel human leukaemic cell line, designated CTS, was established from the peripheral blood of a 13-year-old girl suffering from acute myeloblastic leukaemia (AML) in relapse. Reagents, especially TPA and hemin, induced the expression of a megakaryocytic antigen. It was suggested that CTS cells was less differentiated and have he potentialto differentiate towards multilineage as well as pluripotent stem cells.
2. Tussue specific expression of novel gene product
The antibody raised against novel gene product stained matured megakaryocytes, platelets and reticulocytes in bone marrow. This antibody also stained strongly CMK cells treated with GM-CSF to differentiate to mature megakaryocyte. Thus it is suggested that this gene is specifically expressed in erythro-megakaryocyte lineages.
3. Intracellular localization of novel gene products
The antibody raised against novel gene product stained cytoplasm of CMK cells and human megakaryocytes in bone marrow. It is suggested this gene-product localizes in cytoplasm and has certain role in the platelet production from megakaryocytes and enucleation of erythrocytes.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Yokoe H,: "Induction of polyploidization in the human erythroleukemia cell line(HEL)by the protein kinase inhibitor(K252a)and the phorbol ester-TPA." Leukemia and Lymphoma.25. 333-343 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤武幸、: "Thorombopoietin:シグナル伝達と臨床応用" 日本小児血液学会誌. 11. 397-404 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato T,: "Binding of thrombopoetin to human megakaryocytes and its regulation by thrombopoetin and phorbol ester." British Journal of Hematology.100. 704-711 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miura N: "Estabishment of a new human megakaryoblastic cell line,CMY,with chromosome 17p adnormalities." International Journal of Molecular Medicine. 1. 559-563 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoe H, Masumi A, Sunohara M, Tanzawa H, Sato K, Sato T, Fuse A.: "Induction of polyploidization in the human erythroleukemia cell line (HEL) by the protein kinase inhibitor (K252a) and the phorbol ester-TPA." Leukemia Lymph.25. 333-343 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato T., Fuse A.: "Thrombopoietin : Signal Transduction and Clinical Applications" Jpn.J.Pediatr.Hematol.11. 397-404 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato T, Fuse A, Niimi H, Fielder PJ, Avraham H: "Binding of thrombopoetin to human megakaryocytes and its regulation by thrombopoetin and phrobol ester." Bri.J.Haematol.100. 704-711 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miura N, Sato T, Fuse A, Okimoto Y, Kinugawa N, Horie H, Ota S, Kakuda H, Yokoe H, Miya T, Suzuki N, Niimi H.: "Establishement of a new human megakaryoblastic cell line, CMY,with chromosome 17p abnormalities." Int.J.Mol.Med.1. 559-563 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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