1998 Fiscal Year Final Research Report Summary
Novel genes expressed during megakaryocytic differentiation
| Project/Area Number |
09670245
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
FUSE Akira Laboratory of Physicochemistry and Department of Safety, Research on Biologics, National Institution of Infectious Diseases, Chief, 安全性研究部, 室長 (60110300)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Takeyuki Department of Pediatrics, School of Medicine, Chiba University, Associate Proffe, 医学部, 助教授 (30187207)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Megakaryocyte differentiation / Erythrocyte differentiation / platelet production, enucleation / 脱核 / 巨核化 / 細胞構造変化 |
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| Research Abstract |
1. Establishment of Megakaryoblastic cell line
A novel human leukaemic cell line, designated CTS, was established from the peripheral blood of a 13-year-old girl suffering from acute myeloblastic leukaemia (AML) in relapse. Reagents, especially TPA and hemin, induced the expression of a megakaryocytic antigen. It was suggested that CTS cells was less differentiated and have he potentialto differentiate towards multilineage as well as pluripotent stem cells.
2. Tussue specific expression of novel gene product
The antibody raised against novel gene product stained matured megakaryocytes, platelets and reticulocytes in bone marrow. This antibody also stained strongly CMK cells treated with GM-CSF to differentiate to mature megakaryocyte. Thus it is suggested that this gene is specifically expressed in erythro-megakaryocyte lineages.
3. Intracellular localization of novel gene products
The antibody raised against novel gene product stained cytoplasm of CMK cells and human megakaryocytes in bone marrow. It is suggested this gene-product localizes in cytoplasm and has certain role in the platelet production from megakaryocytes and enucleation of erythrocytes.
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[Publications] Miura N, Sato T, Fuse A, Okimoto Y, Kinugawa N, Horie H, Ota S, Kakuda H, Yokoe H, Miya T, Suzuki N, Niimi H.: "Establishement of a new human megakaryoblastic cell line, CMY,with chromosome 17p abnormalities." Int.J.Mol.Med.1. 559-563 (1998)
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