1998 Fiscal Year Final Research Report Summary
A Role for ch1 in colorectal carcinoma
| Project/Area Number |
09670246
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Metropolitan institute of Medical Science |
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Principal Investigator |
TANAKA Kiyoko Tokyo Metropolitan institute of Medical Science, Dev of tumor biochemistry Researcher, 腫瘍生化学研究部門, 研究員 (40124474)
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| Project Period (FY) |
1997 – 1998
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| Keywords | colorectal carcinama / screening / chromosome 1 / cDNA library / northern blot / tumor suppressor gene / RACE / chromosome introduction |
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| Research Abstract |
Development of colon carcinomas appears to be associated with inactivation of multiple tumor suppressor genes. Cytogenetic and DNA analyses of colon carcinoma have detected a high frequency of chromosome 1p deletion, which suggersts the presence of a tumor suppressor gene. So, we tried the isolation of tumor suppressor gene on chromosome 1 using nontumorigenic colon carcinoma cells containing normal chromosome 1p34-36 and tumorigenic revertantcells.A subtractive cDNA library specific to nontumorigenic colon carcinoma cells was constructed using oligo(dT)30-Latex. As a results of screening, we obtained a clone (G13) which expressed normal chromosome 1p34-36introduced cells and A9chl but not express parental COKFu and revertant cells. Northern bolt analysis revealed that G13 is highly expressed in normal mucosa but reduced in colon carcinomas. Next, we performed northern blot analysis on various human tissues, G13 transcripts were detected in colon, small intestine, ovary, testis, heart, and placenta and the gene evolutionarily conserved. Now, we try the full length gene by the RACE method.
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