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1998 Fiscal Year Final Research Report Summary

Regulation of polyclonal IgE production and supressor mechanisms of Th2 ifilarial parasite infection

Research Project

Project/Area Number 09670254
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionTokyo Medical and Dental University

Principal Investigator

FUJITA Koichiro  Faculty of Medicine, Professor, 医学部, 教授 (90053107)

Co-Investigator(Kenkyū-buntansha) 今井 伸二郎  日清製粉(株)創薬研究所, 主任研究員
TSUKIDATE Setsuko  Faculty of Medicine, Instructor, 医学部, 助手 (40121256)
AKAO Nobuaki  Faculty of Medicine, Lecturer, 医学部, 講師 (00126559)
Project Period (FY) 1997 – 1998
Keywordsnonspecific IgE / Th2 / Dirofilaria immitis / Cytokine / IL-10 / B1 cell
Research Abstract

The filarial parasite infections induced high levels of serum lgE.However, the mechanisms of this phenomenon have not yet been fully elucidated. We have previously reported that the recombinant Dirofilaria immitis-derived protein(DiAg) induced polyclonal lgE production in BALB/c mice, and clarified that the responder cells to DiAg were B cell.
In this experiment, we investigated the mechanisms of polyclonal lgE production by cytokine production and studied the surface molecule expression. The production of IgE, in vitro, was significantly enhanced when restinng splenic B cells were stimulated with the combination of DiAg plus lL-4, but not DiAg alone. DiAg up-regulated the expression of CD23 on splenic B cells in vivo as well as in vitro. Expression of lL-6 and iL-10 mRNAs was relatively higher in DiAg-treated B cells as compared with untreated controls. These results suggested that DiAg enhanced IgE synthesis by inducing autocrine production of lL-6, lL-lO and by directly up-regulating CD23 expression on B cells. Then we were cleared that lL-2 production of Jurkat cells and activated T cells increased with stimulation of DiAg component. DiAg showed various actions as same as cytokine against the host immune systems.

  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Pam Y,Yamada S,Tsukidate S,Fujita K.: "Increased susceptibility of BALB/C mice to infection with Brugia pahaugi when triated at an early stage with a single dose of carrageenan or promethazine." Palasitology International. 47(1). 1-9 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤田 紘一郎: "アレルギーと寄生虫" Allergology (アレルギー科). 6(1). 49-53 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Pan Y,Yamada S,Tsukidate S,Fujita K: "Increased susceptibility of BALB/C mice to infection with Brugia pahangi when treated at an early stage with a single dose of carrageenan and promethajine" Parasitology International. 47(1). 1-9 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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