1998 Fiscal Year Final Research Report Summary
Relationship between regulation of cholera toxin production by lysophosholipase and ToxR.
| Project/Area Number |
09670280
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Okayama Prefectural University (1998)
Osaka University (1997) |
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Principal Investigator |
YAMAMOTO Koichiro Okayama Pref.Univ., Fac.Health Welfare Sci., Professor, 保健福祉学部, 教授 (30158274)
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| Co-Investigator(Kenkyū-buntansha) |
ARITA Michiko Okayama Pref.Univ., Fac.Health Welfare Sci., Research Associate, 保健福祉学部, 助手 (10127178)
HONDA Takeshi Osaka Univ., Res.Inst.Microbial Dis., Professor (60029808)
IIDA Tetsuya Osaka Univ., Res.Inst.Microbial Dis., Associate Professor (90221746)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Phospholipase / Vibrio cholerae / toxin / Gene / choromosome / lypA / ctxAB / toxR |
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| Research Abstract |
By pulse-field electrophoresis and Southern hybridization, we found the lypA band toxR geneswere in the same NotI DNA fragment of Vibrio cholerae chromosome. This suggest close proximity between lypA and toxR.
Insertional mutants in the lypA gene was constructed using suicide vector containing inner fragment of the lypA gene. Cholera toxin-producing ability was reduced in these insertional mutants, especially in the strain which highly produces the toxin, such as strain 569B.In the insertional mutant strain which reduced production of cholera toxin, transcription of toxR was also down-regulated.
We deleted the lypA gene completely by double-crossover recombination. In this deletion mutant, production of cholera toxin and transcription of toxR were not reduced clearly. This suggest that insertion of plasmid into the lypA in the insertional mutant may affect the neighboring genes, which affect production of cholera toxin.
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[Publications] Makino, K., Ishii, K., Yasunaga, T., Hattori, M., Yokoyama, K., Yutsudo, C., Kubota, Y., Yamaichi, Y., Iida, T., Yamamoto, K., Honda, T., Han, C-G., Ohtsubo, E., Kasamatsu, M., Hayashi, T., Kuhara, S.and Shinagawa, H.: "Complete nucleotide sequences of 98-kb and 3.3-kb plasmids of an enterohemorrhagic Escherichia coli O157 : H7 derived from Sakai outbreak" DNA Res. 5. 1-9 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Iida, T., Park, K-S., Suthienkul, O., Kozawa, J., Yamaichi, Y., Yamamoto, K., Honda, T.: "Close proximity of the tdh, trh and ure genes on the chromosome of Vibrio parahaemolyticus." Micriobiology. l44. 2517-2523 (1998)
Description
「研究成果報告書概要(欧文)」より
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