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1998 Fiscal Year Final Research Report Summary

Physiological aspects of cholesteryl glucosides in Helicobacter pylori

Research Project

Project/Area Number 09670281
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionJichi Medical School

Principal Investigator

HIRAI Yosikazu  Jichi Medical School, Dept.of Microbiology, Professor, 医学部, 教授 (00127581)

Co-Investigator(Kenkyū-buntansha) YOKOTA Kenji  Okayama Medical School, Dept.of Bacteriology, Assistant Professor, 医学部・細菌学, 助手 (00243460)
Project Period (FY) 1997 – 1998
KeywordsHelicobacter pylori / Cholesteryl glucosides / Lipids / Cholesterol / Growth phases / Coccoid form
Research Abstract

We have detected three types of cholesteryl glucosides (CGs) in H.pylofl (HP) cultured in a serum-supplemented medium. The structures of the CGs were cholesteryl glucoside (CGL), acylated CGL (CAG), and phosphatidyl CGL (CPG).
Firstly, we investigated bacterial growth in a serum-supplemented medium with shaking under microaerophlic conditIon using with a chamber (Concept plus, Ruskinn tech., UK). Under this condition, subculture and culture of HP can be done without exposing usual air.
We investigated the lipid, especially CGs, compositions of HP in each bacterial growth phase under the condition mentioned above. In CGs, CGL and CAG were detected at all growth phases. However, little of CPG was detected at exponential phase. CPG was detected In spiral form bacteria of stationary phase, and its content dramatically increased at declining phase (phase of coccoid formation), whereas the content of phosphatidyl ethanolarnine, main phosphoilpid of HP, was dramatically decreased at declining phase. The metabolism of phosphate was appeared to be dramaticaliy changed after stationary phase in HP.
We investigated the uptakes of sterols and those related compounds, and the synthesis of glucosides from those in a serum-free medium under the same condition mentIoned above. HP accumulated free cholesterol, ergosterol, stigmasterol, beta-sitosterol, and synthesize glucosides from those. However, HP did not accumulate cholesterol ester. Therefore, the structure of 3 beta-ol could be important for the accumulation of sterol. HP accumulated vitamin D3, cholecalciferol, but did not synthesize glucoside from it. For the synthesis of glucosides after the accumulation, the base structure of sterol would be important. Furthermore, vitamin D3 inhibited the growth of HP, and its MIC against HP In a serum-free medium was 12.5 mug/ml. The hydrophobic substances which are accumulated but not synthesized to glucosides such as vitamin D3 may inhibited the growth in HP.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] HIRAI, Y.: "The biosynthesis of cholesteryl glucosides in Helicobacter pylori" Gut. 41(suppl.1). All (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HAYASHI,S.: "Effect of escabet sodium on Helicobacter pylori adhesion to gasric epithelial cells" J.Gastroenterol.32. 593-597 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HAYASHI, S.: "A rapid and simple methods to quantify Helicobacter pylori adhesion to human gastric MKN-28 cells" J.Gastroenterol.Hepatol.12. 373-375 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KOBAYASHI, K.: "Detection of Helicobacter pylori associated antigen and heat shock protein 60 on folliculor dendritic cells inthe ……" J.Clin.Paehol.51. 396-398 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HAYASHI, S.: "Analysis of Immunogloblin A antibodies to Helicobacter pylori in serum and gastric juice in relation to mucosal inflamation" Clin.Didg.Lab.Immunol.5. 617-621 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HAYASHI,S.: "Modification of Helicobacter pylori adhesion to human gastric epiehelial cells by antiadhesion agents" Diag.Dis.Sci.43(suppl.). 56s-60s (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平井 義一: "ウィルス・細菌感染newファイル(ヘリコバクターを分担)" 羊土社, 東京, 243 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Hirai, et al.: "The biosynthesis of cholesteryl glucosides in Helicobacter pylori" Gut. 41 (suppl.1). A11 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Hayashi, et al.: "Effect of escabet sodium on Helicobacter pylori adhesion to gastric epithelial cells." J.Gastroenterol.32. 593-597 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Hayashi, et al.: "A rapid and simple method to quantify Helicobacter pylori adhesion to human gastric MKN-28 cells." J.gasroenterol.Hepatol.12. 373-375 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kobayashi, et al: "Detection of Helicobacter pylori associated antigen and heat shock protein 60 on follicular dendritic cells in the germinal centres of low grade B cell lymphoma of gastric mucosa associated lymphoid tissue (MALT)." J.Clin.Pathol.51. 396-398 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Hayashi, et al.: "Analysis of Immunoglobulin A antibodies to Helicobacter pylori in serum and gastric juice in relation to mucosal inflammation." Clin.Diag.Lab.Immunol.5. 617-621 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Hayashi, et al.: "Modification of Helicobacter pylori adhesion to human gastric epithelial cells by antiadhesion agents." Dig.Dis.Sci.43 (suppl.). 56S-60S (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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