| Co-Investigator(Kenkyū-buntansha) |
HAMAJIMA Kenji Yokohama City University, Department of Bacteriology, Instructo, 医学部, 助手 (00114611)
AOKI Ichirou Yokohama City University, Department of Pathology, Professor, 医学部, 教授 (00184028)
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| Research Abstract |
The efficacy of using pLasmid DNA-encoding microbial antigens to by us raise humoral and cellular immune responses against pathogens has been reported in several animal models, and this method is referred to as DNA vaccination. In the field of AIDS vaccine development, others and we have succeeded in inducing HIV-1-specific immune responses by DNA vaccination. DNA vaccination offers potential advantages over traditional protein-based vaccines in areas such as stability, ease of production, and induction of cell-mediated immunity. To obtain stronger responses, approaches such as the use of particle bombardment by means of a gene gun system, or application of local anesthetics to facilitate DNA entry into muscle cells have been attempted and have met with substantial success.
In the present series of series of studies we have demonstrated that 1) DNA vaccine can induce a high level of Th1 immune responses, 2) and these responses are enhanced by the addition of IL-12 expression plasmids. 3) GM-CSF expression plasmids enhanced Th2 type immune responses. 4) Intranasal administration (i.n.) of DNA vaccine enhance induced higher levels of Th2 immune responses than intramuscular immunization. 5) In administration of DNA vaccine induced high levels of mucosal immunities. In this series of our studies revealed that DNA vaccine could induce a good level of antigen specific immune responses.
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