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1999 Fiscal Year Final Research Report Summary

Analysis of a fuctor involving trnslocation of LT to the cell.

Research Project

Project/Area Number 09670302
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionFujita Health University

Principal Investigator

TSUJI Takao  Fujita Health University. School of Medicine, Microbiology Professor, 医学部, 教授 (60171998)

Project Period (FY) 1997 – 1999
KeywordsETEC / Entocytosis / GM1 ganglioside / Enterotoxin (LT)
Research Abstract

We have been analyzing whether there might be a factor involving the endocytosis of ETEC enterotoxin (LT) to target cell except GMI ganglioside receptor. After LT was added to the CHO cell, Immunoprecipitates with anti-LT antibody to cell lysate was analyzed with Western blotting with anti-LT antibody. There was a band, of which molecular weight was 22,000 except the monomer of LT-B and might shift the monomer upper side of gel. Then we examined this factor. However, some other examiner reported that this shift of the monomer was caused by the formation of dimer of LT-B in the cell. Therefore, we examined whether other factor might be involved in the endocytosis of LT to make an antibody blocking the endocytosis of LT-B.
Some rabbits were immunized by cell lysate, which was prepared from NCTC 2071 defecting GM1 ganglioside and we can prepared the anti-serum partially blocking the internization of LT to CHO cells. Then, we are now examining a factor reacting to this antiserum.
Morecover, while we received this grant, we reported data as followed;
1. LT is also coded in the chromosome, except the plasmid.
2. The mutant defecting nicking site of A subunit near Arg 192 have strong adjuvant action to VZV, KKLH, Ovalbumin, BGG and measle virus.
3. The nicking site near Arg 192 of the A subnit is not involved in some biological activity of LT.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Tsuji et al: "Relationship between a low toxicity of the mutant A subunit of ETEC enterotoxin ……"Immunology. 90. 176-182 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato et al: "The major component of Na-CBZ-L-lysine thiobenzlester(BLT)-specific ******** ……"Immunobiology. 196. 465-474 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuji et al: "ETEC enterotoxin demonstrates partial toxicity independent of the nicking Arg192"Microbiology. 143. 1797-1804 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuji et al: "Induction of apoptosis in lymphoid tissues of mice after intramuscular infection of ETTEC enterotoxin"Immunobiology. 201. 377-390 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Homma et al: "Effect of EM on Shigella infection of Caco-2 cells"FEMS Immunol and Medical Microbiol. 27. 139-145 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuji et al: "Humoral immunoresponse to VZV pernasally coadministered with E coli cuterotoxin in mice"Vaccine. 18(19). 2049-2054 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Imamura S., Kido N., Kato M., Kawase H., Miyama A., and Tsuji T.: "An unique DNA sequence of human enterotoxigenic Escherichia coli enterotoxin encoded by chromosomal DNA"FERM. Microbiol.Lett.. 146. 241-245 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato M., Imamura S., Kawase H., Miyama A., and Tsuji T.: "Histidine-44 of the A subunit of Escherichia coli enterotoxin is involved in its enzymatic and bilological activities."FEMS. Microbiol. Lett.. 152. 219-225 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuji T., Kato M., Kawase H., Imamura S., Kamiya H., Ichinose Y. and Miyama A.: "Escherichia coli enterotoxin demonstrates partial toxicity independent of the nicking around Arg192"Microbiology. 143. 1797-1804 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuji T., Yokochi T., Kamiya H., Kawamoto K., Miyama A., and Asano Y.: "Relationship between a low toxicity of the mutant A subunit of enterotoxigenic Escherichia coli enterotoxin and its strong adjuvant action"Immunology. 90. 176-182 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasaki K., Kobayashi T., Imamura S., Shigekura T., Kado R., Kawamoto K., Tsuji T. and Miyama A.: "Flow cytometry analysis of the Fas ligand expression of activated lymph node T-cells"Immunol.Lett.. 55. 1-13 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuji T, Asano Y, Handa T, Honma Y, Ixhinose Y and Yokochi T: "In vivo induction of apoptosis in mouse lympoid tissues by indirect effect of enterotoxigenic Escherichia Coli enterotoxin."Immunobiology.. 201. 377-390 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honma Y, Sasakawa C, Tsuji T, and Iwanaga M.: "Effect of erythromycin on Shigella infection of Caco-2 cells"FEMS Immunology and Medical Microbiology.. 27. 139-145 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuji T, Shiraki K, Sato H, Yue-Mea J, Honma Y, Yoshikawa T and Asano Y.: "Humoral immunoresponse to varicella-zoster virus pernasally coadministered with Escherichia coli enterotoxin in mice."Vaccine.. 18. 2049-54 (2000)

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      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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