STUDIES ON MOLECULAR PATHOGENICITY OF PARAMYXOVIRUS

1999 Fiscal Year Final Research Report Summary

• Project/Area Number: 09670322
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: NATIONAL INSTITUTE OF INFECTIOUS DISEASES
• Principal Investigator: YAMADA Akio NATIONAL INSTITUTE OF INFECTIOUS DISEASES, TSUKUBA PRIMATE CENTER FOR MEDICAL SCIENCE, DIRECTOR, 筑波医学実験用霊長類センター, センター長 (50150876)
• Co-Investigator(Kenkyū-buntansha): TANABAYASHI Kiyoshi NATIONAL INSTITUTE OF INFECTIOUS DISEASES, TSUKUBA PRIMATE CENTER FOR MEDICAL SCIENCE, SENIOR SCIENTIST, 筑波医学実験用霊長類センター, 主任研究官 (50197505) ; TAKEUCHI Kaoru NATIONAL INSTITUTE OF INFECTIOUS DISEASES, DEPARTMENT OF VACCINE CONTROL AND VIRAL DISEASES, SENIOR SCIENTIST, ウイルス製剤部, 主任研究官 (00192162)
• Project Period (FY): 1997 – 1999
• Keywords: PARAMYXOVIRUSES / MUMPS VIRUS / CELL FUSION / PATHOGENICITY / REVERSE GENETICS

Research Abstract

In order to understand the molecular mechanisms underlying paramyxovirus pathogenesis, we have tried to clarify the role of genes encoding glycoproteins as well as to establish the method for the recovery of infectious viruses from cloned viral genomes. Through the characterization of mumps virus stains exhibiting different fusion activity, we have shown that the amino acid at position 262 was crucial in terms of determining the extent of induction of cell fusion. We have also immunized mice with plasmid DNA containing the F gene of mumps virus and found that the immunized mice produced antibody against the F protein. Although it is difficult to obtain anti-F antibody that can neutralize viruses, this immunization protocol was quite efficient to induce neutralizing anti-F antibody. Moreover we have succeeded to produce infectious measles viruses by transfection of plasmid DNA containing cDNA corresponding the entire measles virus genome according to the method established by Billter et al. This experience seems extremely important in establishing the reverse genetics for mumps virus.

Research Products

• [Publications] K Takeuchi, N Miyajima, F Kobune, and Tashiro: "Comparative nucleotide sequence analyses of the entire genomes of B95a cell-isolated and Vero cell-isolated measles viruses from the same patient"Virus Genes. (印刷中). (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T Fukao, A Hirano, K Nakamura, Y Yamazaki, A Yamada, H Tashita, R Inoue , N kondo: "Perinatal mumps associated with bronchiolitis and respiratory distress"Eur J Pediatr. 157. 952-953 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Saito, H Takahashi Y, Harata S, Tanaka K, Sato H, Suto T, Yamada A, Yamazakii S, Morita M.: "Cloning and characterization of the genomic RNA sequence of the mumps virusstrain associated with a high incidence of aseptic meningitis"Microbiol Immunol. 42. 133-137 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ohsawa K, Yamada A, Takeuchi K, Watanabe Y, Miyata H, Sato H.: "Genetic characterization of parainfluenza virus 3 derived from guinea pigs"J Vet Med Sci. 60. 919-922 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takeuchi, K., Miyajima, N., Kobune, F., and Tashiro, M.: "Comparative nucleotide sequence analysis of the entire genomes of B95a cell-isolated and Vero cell-isolated measles viruses from the same patient."Virus Genes,. (in press). (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Fukao, T., Hirano, K, Yamazaki, Y., Yamada, A., Tashita H., Inoue R., Kondo, N: "Perinatal mumps associated with bronchiolitis and respiratory distress"Eur J Pediatr.. 157. 952-953 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Saito H, Takahashi Y, Harata S, Tanaka K, Sato H, Suto T, Yamada A, Yamazaki S, Morita M.: "Cloning and characterization of the genomic RNA sequence of the mumps virus strain associated with a high incidence of aseptic meningitis."Microbiol Immunol,. 42. 133-137 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ohsawa K, Yamada A, Takeuchi K, Watanabe Y, Miyata H, Sato H.: "Genetic characterization of parainfluenza virus 3 derived from guinea pigs"J Vet Med Sci,. 60. 919-922 (1998)
  • Description: 「研究成果報告書概要(欧文)」より