1998 Fiscal Year Final Research Report Summary
Studies on the mechanisms of growth and diffrentiation of pre-T cells
| Project/Area Number |
09670336
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
KINA Tatsuo Kyoto University, Institute for Frontier Medical Sciences, Associate Professor, 再生医科学研究所, 助教授 (30127071)
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| Project Period (FY) |
1997 – 1998
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| Keywords | pre-T cell / cell differentiation / thymus / signal transduction / G-protein / integrin |
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| Research Abstract |
The present study aimed to explore the molecular mechanisms of growth and differentiation of early T cells in the thymus. For this purpose, we introduced a stromal-cell dependent pre-T cell clone (BTK-24) as an in vitro model for pre-T cell development. BTK-24 is a typical pre-T cell clone with a rearranged TCRbeta gene and germline TCRalpha genes. Growth of BTK-24 requires direct cell contact with stromal cells, although a kind of solube mediator(s) released from stromal cells is also involved for optimal growth. We found that adhesion between BTK-24 and stromal cells is mediated by VLA-4/VCAM-1 interaction. The soluble mediator involved in pre-T cell growth appears to be a small chemokine-like molecule, because its activity was lost by dialysis, and because stimulation with IL-1, TNFalpha or LPS greatly increased the growth promoting activity of the mediator. A low dose of pertussis toxin (PTX) is highly suppressive for BTK-24 growth, suggesting that inhibitory G-protein alpha (Galphai )-mediated signal pathway is important for growth. It was also demonstrated that co- culture of TK-24 with primary thymic stromal cell monolayer resulted in the induction of CD3/TCR complex and CD4 antigen on BTK-24 cells. These results suggest that both direct (adhesion) and indirect (cytokine) interactions 'with stromal cells are important for pre-T cell growth, and that some stromal cells in the thymus has ability to induce the differentiation of pre-T cells.
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Research Products
(10 results)
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[Publications] Satoh, A., Takayama, E., Kojima, K., Ogawa, H., Katsura, Y., Kina, T., and Matsumoto, I.: "Characterization of human p33/41 (annexin IV), a Ca2+ dependent carbohydrate- binding protein with monoclonal anti-annexin IV antibodies, AS11 and AS17." Biol.Pharm.Bull.20 (3). 224-229 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Satoh, A., Takayama, E., Kojima, K., Ogawa, H., Katsura, Y., Kina, T., Irimura T., and Matsumoro, I.: "Modulation of cell surface lectin receptors on K562 human erythroleukemia cells induced by transfection with annexin IV cDNA" FEBS Letter. 405 (1). 107-110 (1997)
Description
「研究成果報告書概要(欧文)」より
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