1998 Fiscal Year Final Research Report Summary
SEARCH AND IDENFICATION OF NOVEL LIGANDS FOR SELECTINS
| Project/Area Number |
09670345
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | INSTITUTE OF LIFE SCIENCE,SOKA UNICERSITY |
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Principal Investigator |
NARIMATSU Hisashi INSTITUTE OF LIFE SCIENCE,SOKA UNIVERSITY,PROFESSOR, 生命科学研究所, 教授 (40129581)
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| Co-Investigator(Kenkyū-buntansha) |
KUDO Takashi INSTITUTE OF LIFE SCIENCE,SOKA UNIVERSITY,INSTRUCTOR, 生命科学研究所, 助手 (20288062)
NISHIHARA Shoko INSTITUTE OF LIFE SCIENCE,SOKA UNIVERSITY,ASSOCIATE PROFESSOR., 生命科学研究所, 助教授 (00164575)
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| Project Period (FY) |
1997 – 1998
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| Keywords | GLYCOSYLTRANSFERASE / FUCOSYLTRANSFERASE / SELECTIN / CANCER / GALACTOSYLTRANSFERASE / LEWIS ANTIGEN / CARBOHYDRATE / CA19-9 |
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| Research Abstract |
We searched and identified for new members of glycosyltransferases involved in the synthesis of Lewis antigens which are the ligands for selectins. Successfully, we have cloned two novel genes encoding new glycosyltransferases, and named Fuc-TIX and beta3Gal-T5. Fuc-TIX was mainly expressed in brain and kidney, and participated in the synthesis of CD15 (Le^x) antigens. beta3Gal-T5 was found to be responsible for the synthesis of type 1 Lewis antigens, such as Le^a, Le^b and sLe^a. In particular, B3GaI-T5 was proven to be a responsible enzyme for the synthesis of CA 19-9 (sLe^a) antigen in gastrointestinal tissues. CA 19-9 is a very famous tumor marker, and its carbohydrate structure is defined as sLe^a which is the selectin ligand.
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[Publications] Kimura, H., Shinya, N., Nishihara, S., Kaneko, M., Irimura, T.and Narimatsu, H.: "Distinct substrate specificities of five human alpha-1,3-fucosyltransferases for in vivo synthesis of the sialyl Lewis x and Lewis x epitopes." Biophys.Biochem.Res.Commun.237. 131-137 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Narimatsu.H., Iwasaki, H., Nakayama, F., Ikehara, Y., Kudo, T., Nishihara, S., Sugano, K., Okura, H., Fujita, S.and Hirohashi, S.: "Lewis and Secretor gene dosages affect CA19-9 and DU-PAN-2 serum levels in normal individuals and colorectal cancer patients." Cancer Res.58. 512-518 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kudo, T., Ikehara, Y., Togayachi, A., Morozumi, K., Watanabe, M., Nakamura, M., Nishihara, S.and Narimatsu, H.: "Upregulation of a set of glycosyltransferase genes in human colorectal cancer" Lab.Invest.7. 797-811 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Kudo, T., Ikehara, Y., Togayachi, A., Kaneko, M., Hiraga, T., Sasaki, K.and Narimatsu, H.: "Expression cloning and characterization of a novel murine alpha1,3-fucosyltransferase, mFuc-TIX,that synthesizes the Lewis x (CD15) epitope in brain and kideny." J.Biol.Chem.273. 26729-26738 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Nakamura, M., Kudo, T., Narimatsu, H., Furukawa, Y., Kikuchi, J., Asakura, S., Yang, W., Iwase, S., Hatake, K., Miura, Y.: "Single glycosyltransferase, core 2beta-1,6-N-acetylglucosaminyltransferase, regulates cell surface sialyl-Lex expression level in human pre-B lymphocytic leukemia cell line KM3 treated with phorbolester." J.Biol.Chem.273. 26779-26789 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nakamura, M., Furukawa, Y., Sasaki, R., Masuyama, Ji, Kikuchi, J., Iwase, S., Kudo, T., Narimatsu, H., Asakura, S., Fujiwara, S.and Inokuchi, Ji.: "UDP-GlcNAc : Galbeta1-3GalNAc (GlcNAc to GalNAc) beta1-6N-acetylglucosaminyltransferase holds a key role on the cntrol of CDs expression in human pre-B lymphoid cell lines." Glycobiology. 9. 1-12 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Nishihara, S., Hiraga, T., Ikehara, Y., Iwasaki, H., Kudo, T., Yazawa, S., Morozumi, K., Suda, Y.and Narimatsu, H.: "Molecular behavior of mutant Lewis enzymes in vivo." Glycobiology. 9. 373-382 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Issiki, S., Togayachi, A., Kudo, T., Nishihara, S., Watanabe, M., Kubota, T., Kitajima, M., Shiraishi, N., Sasaki, K., Andoh, T.and Narimatsu, H.: "Cloning, expression and characterization of a novel UDP-galactose : beta-N-acetylglucosamine beta 1,3-galactosyltransferase (beta3Gal-T5) responsible for synthesis of type 1 chain in colorectal and pancreatic epithelia and tumor cells derived therefrom." J.Biol.Chem.274. 12499-12507 (1999)
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「研究成果報告書概要(欧文)」より
