1998 Fiscal Year Final Research Report Summary
The study of HLA-DNA typing technique applicable to forensic investigation
| Project/Area Number |
09670434
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Shinshu University |
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Principal Investigator |
OTA Masao Shinshu University School of Medicine, Department of Legal Medicine, Lecturer, 医学部, 講師 (50115333)
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| Project Period (FY) |
1997 – 1998
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| Keywords | HLA / Class I genes / Class II genes / Forensic samples / DNA typing / PCR-SSP / PCR-RFLP / nested PCR |
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| Research Abstract |
I investigated the applicability of HLA -DNA typing using PCR-based technique to forensic investigations. First, I applied the HLA-class II typing combined With PCR-SSP and PCR-RFLP to forensic practice. PCR-SSP typing was an effective as serological HLA-DR typing in determining DR types. PCR-RFLP typing was used for more precise definition of DRB1 alleles encoding DR2, DDR4, and DR8. Furthermore I used the nested or semi-nested PCR technique to increase the sensitivity of this system. Second, I studied the applicability of HLA-class I typing using PCR-SSP method. I made 96 kinds of primer mixture to amplify HLA-A,-B and-C alleles corresponding to serological class I antigens.
HLA-DNA typing by these methods were possible from DNA samples extracted from forensic samples, but both quality and quantity of DNA were important for successful.
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[Publications] Ota M., Katsuyama Y., Mizuki N., Ando H., Furihata K., Ono S., pivetti-Pezzi P., Tabbara K., Palimeris G.D., Nikbin B., Davatchi F., Chams H., Geng Z., Bahram S., Inoko H.: "Tirnucleotide repeat polymorphism wihtin exon 5 of the MICA gene (MHC class I chain-related gene A) : allele frequency data in the nine population groups Japanese, Northern Han, Hui, Uygur, Kazakhstan, Iranian, Saudi Arabian, Greek and Italian." Tissue-Antigens. 49. 448-454 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ota M., Arakura A., Liu C.Y., Takayanagi K., Fukushima H., Katsuyama Y., Tamiya G., Shiina T., Oka A., Inoko H.: Microsatellite polymorphism in the HLA class I region. (Japanese), DNA polymorphisms, Vol6, I.Nakazono ed.Toyo Shoten, Tokyo, 162-165 (1998)
Description
「研究成果報告書概要(欧文)」より
