| Research Abstract |
A accurate and rapid typing system at three STR loci with fluorescently labeled primers was devised using a convenient DNA extraction kit from bloodstains, and this work was published in the Japanese Journal of Legal Medicine in 1997. In collaboration with a company, PE Applied Biosystem, we made a database of 9 STRs (D3S1358, vWA, FGA, TH01, TPOX, CSF1PO, D5S818, D13S317, D7S820) in 206 of Japanese with the commercially available kit, AmpFlSTR Profiler kit. This kit makes it possible to type 9 STRs by multiplex PCR using multi-colored fluorescent dyed primers. We calculated allele frequencies and some statistical values for personal identification and paternity testing at 9 STR loci. The result of this work was presented in the 17th International Society of Forensic Haemogenetics in Oslo in 1997, and published in Journal of Forensic Sciences in 1999. In 1998, we calculated allele frequencies in 207 of Japanese at 13 STR loci, 9 loci mentioned above and other 4 loci (D18S51, D21S11, D8
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S1179, D16S539), with the AmpFlSTR Profiler plus kit and the Cofiler kit. Since foreigners, especially Asian populations are occasionally concerned the crimes in Japan currently, we also investigated allele frequencies at those 13 STR loci in 118 of Chinese (Beijing area), 122 of Burmese and 110 of Korean. As the result of this work, it was suggested this system of 13 STRs has also extremely high power of discrimination in each population, and each population has a few STR loci significantly different from Japanese in pairwise comparison of allele distribution at each loci. Furthermore, various tests for Hardy-Weinberg equilibrium were evaluated using Japanese allele frequencies at 9 STR loci, and the results were summarized and published in DNA Polymorphisms.
We applied the AmpFlSTR Profiler kit to a paternity testing, so-called an isolated exclusion case, in which the biological fatherfood was excluded at only one locus (D3S1358 in this case) in 15 loci examined. It was confirmed that the exclusive allele at D3S1358 was derived from paternal mutantion using MVR-PCR at D1S8 and D7S21. This work was presented in the 82nd annual meeting of Japanese Society of Legal Medicine. We also applied this system to the personal identification of decomposed cadavers using their toothbrushes kept in houses as reference samples. Less
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