1998 Fiscal Year Final Research Report Summary
Evaluation of Enzyme Induction in Alcohol Metabolism by using RT-PCR.
| Project/Area Number |
09670437
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Mie University |
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Principal Investigator |
FUKUNAGA Tatsushige Mie University, Faculty of Medicine, Professor, 医学部, 教授 (70156800)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Hidetaka Mie University, Faculty of Medicine, Assistant, 医学部, 助手 (90158296)
TANEGASHIMA Akio Mie University, Faculty of Medicine, Lecture, 医学部, 講師 (70283520)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Alcohol, ethyl / Acetaldehyde / ALDH / ADH / cDNA / mRNA / PT-PCR / Polymorphism |
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| Research Abstract |
It is well known that the Oriental flushers, homozygotically possessingALDH2*2, show dysphoric symptoms after intake of even small doses of ethanol. Heterozygotic subjects (ALDH2*2/*1) can, however, drink a small amount and then after a few hours drink a little more alcohol, with the dysphoric symptoms diminish somewhat to the second challenge. The main purpose of this study was to determine whether the levels of mRNA for ALDH2 are different before and after ethanol intake.
A method for determining mRNA levels for ALDH2 in peripheral blood was developed using an RT-PCR technique. DNA-free total RNA was extracted from peripheral lymphocytes from healthy Japanese volunteers. Purified RNA was reverse transcribed into cDNA.The cDNA was amplified with PCR using the primers, designed through exon 11 and 12-13. PCR DNA products were
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Research Products
(12 results)
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[Publications] FUKUNAGA,T., ABE,S., YAMAMOTO,Y., YAMAMOTO,H., TANEGASHIMA,A.and SAIJOH,K.: "Evaluation of mRNA levels for ALDH2 in human peripheral blood before and after ethanol intake." Alcohol Clin Exp Res. 22 (3, Suppl). 188A (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] ABE,S., YAMAMOTO,Y., SAIJOH,K., YAMAMOTO,H., TANEGASHIMA,A.and FUKUNAGA,T.: "ALDH2 mRNA levels in human peripheral lymphocytes." Jpn J Alcohol Studies & Drug Dependence. 33 (4). 500-501 (1998)
Description
「研究成果報告書概要(欧文)」より
