1999 Fiscal Year Final Research Report Summary
DNA analysis of HLA class II gene and application of forensic specimens
| Project/Area Number |
09670452
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Keio University |
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Principal Investigator |
YANAGIDA Junichi Keio University, Legal Medicine, School of Medicine, Professor, 医学部・法医学, 教授 (70049790)
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| Co-Investigator(Kenkyū-buntansha) |
HARA Masaaki The Saitama Medical School, Legal Medicine, Research Assistant, 法医学, 助手 (50129160)
MURAI Tatsuya Keio University, Legal Medicine, School of Medicine, Associate Professor, 医学部・法医学, 助教授 (80129692)
SHINOZUKA Tatsuo Keio University, Legal Medicine, School of Medicine, Assistant Professor, 医学部・法医学, 講師 (70095610)
TOMITA Akiyo Keio University, Legal Medicine, School of Medicine, Research Assistant, 医学部・法医学, 助手 (10286445)
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| Project Period (FY) |
1997 – 1999
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| Keywords | HLA class II gene / DNA polymorphism / paternity testing / direct PCR |
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| Research Abstract |
1) HLA DQA 1 and HLA DRB genotypings by the PCR-SSP and PCR-SSO methods were applied to the cases of disputed paternity. Conventional blood group testing and HLA class II genotyping (HLA DQA 1 and HLA DRB) were good agreement with the caes of paternity test.
2) In field of forensic medicine, biological samples such as bloodstain, semen, and hair are very important samples for personal identification. It has to spend much time and make many effects to extract DNA from torensic samples. The Drop PCR Kit for human blood, by which PCR reaction directly carried out on whole blood and bloodstain without DNA extraction process, was applied to the analyses of HLA DQα and HLA DRB geontypings. The experimental results suggested that HLA class II genotyping from small amount or bloodstain (size: 1mm x 1mm) by direct PCR could be applied to forensic practices.
3) The DNA extracted from the nails of human living bodies and cadavers was examined to clarify its usefulness in the practical analysis of DNA polymorphisms. No significant differences were shown in the recovery and the purity of five different methods (phenol-chloroform) method, SMITEST DNA extraction kit, Wako DNA Extractor WB Kit, DnaQuick and SepaGene) for DNA extraction from nails of living bodies. The DNA from cadaveric nails was able to be extracted by phenol-chloroform method. The applicability of the human nail DNA for the polymorphism analyses with HLA DQ α typing, HLA-DRB typing and PM-PCR typing suggested that the DNA extracted from human nails (both living bodies' and cadavers') is useful the practical test in forensic science.
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[Publications] Kato,M., Shnozuka,T., Sugiuura,K., Takei,S., Murai,T., Yanagida,J., Hara,M and Watanabe,H.: "DNA analysis of cadaveric finger nail"Res.Pract.Forens.Med.. 39. 35-38 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sugiura,K., Shinozuka,T., Kato,M, Takei,S, Murai,T., Yanagida,J., Hara,M. and Watanabe,H.: "A case of paternity test or Chinese Taipei."Res.Pract.Forens.Med.. 39. 45-49 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hara,M,, Saito,K., Takada,A., Kuroda,N., Yabe,K., Watanabe,H,, Shinozuka,T. and Murai,T.: "HLA DQ α gene polymorphism of SIDS."Medicine & Biology. 134(4). 117-121 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kato,M., Shinozuka,T., Takei,S., Murai,T., Yanagida,J., Sugiuura,K., Hara,M. and Watanabe,H.: "Analysis of HLA DQA 1 gene by PCR-SSP method and its application to paternity test."DNA polymorphism. 5. 250-253 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shinozuka,T., Kato,M., Hara,M., Murai,T., Takei,S., Kuroda,N., Watanabe,H. and Yanagida,J.: "Analysis of human nail DNA."Advances in Research on DNA Polymorphisms. 299-302 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sugiura,K., Shinozuka,T., Takei,S., Takada,T., Yahagi,N., Tomita,A., Murai,T. and Yanagida,J.: "Direct amplification method on blood with AmpdirectィイD1TMィエD1 by PCR and HLA class II genotyping."Res.Pract.Forens.Med.. 40. 13-17 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shinozuka,T,, Takada,T., Sugiura,K., Ohue,O., Takei,S., Nakajima,R., Ro,A., Baba,M., Tomita,A., Murai,T., Yanagida,J., Yamamoto,Y., Hara,M. and Watanabe,H.: "DNA typing of blood stain specimens by PCR method with AmpdirectィイD1TMィエD1 reagent."6th Indo Pacific Congress on Legal Medicine and Forensic Sciences. 739-742 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shinozuka,T., Hara,M., Nakajima,R., Takei,S., Ohue,O., Yamamoto,Y., Ro,A., Tomita,A., Murai,T., Watanabe,H. and Yanagida,J.: "HLA DRB genotyping with SSO probes and it's a application to paternity testing."Res.Pract.Forens.Med.. 41. 47-50 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sugiura,K., Shinozuka, T., Takei, S., Takada,T., Nakajima, R., Ro,A., Tomita,A., Murai,T., Yanagida,J., Hara,M. and Watanabe,H.: "DNA typing or blood stain specimens by PCR method with AmpdirectィイD1TMィエD1 reagent."DNA polymorphism. 6. 72-75 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shinozuka,T., Nakajima,R., Ro,A., Baba,M,, Yamamoto,Y., Nakamura,A., Takei,S., Ohue,O., Hisashi,Y., Muarai,T. and Yanagida,J.: "HLA DQα and HLA DRB genotyping of bloodstain by direct-PCR method."Res.Pract.Forens.Med.. 42. 37-40 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamamoto,Y., Kameyama,H., Shinozuka,T., Ohue,O., Takei,S., Nakajima,R., Murai,T. and Yanagida,J.: "DNA typing with Drop PCR Kit for Human BloodtィイD1TMィエD1"DNA polymorphism. 7. 75-78 (1999)
Description
「研究成果報告書概要(欧文)」より
