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1998 Fiscal Year Final Research Report Summary

Molecular analysis of new herpesvirusinfections

Research Project

Project/Area Number 09670477
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionEhime University

Principal Investigator

YASUKAWA Masaki  Ehime University School of Medicine Associate Professor, 医学部, 助教授 (60127917)

Project Period (FY) 1997 – 1998
KeywordsHerpesviruses / HHV-6 / HHV-7 / KSHV / HHV-8 / Apoptosis / CD4 / CXCR4
Research Abstract

Human herpesvirus 6 (HHV-6), HHV-7 and KSHV (HHV-8) are new human herpesviruses which have been recently isolated. In the present study, the pathogenesis of these herpesvirus infections was investigated focusing on the functional alteration of virus-infected CD4+T lymphocytes. Results obtained from the series of the present study are as follows :
1. HHV-6 infection of CD4+T lymphocytes resulted in their apoptosis. The degree of apoptosis increased when HHV-6-inoculated cells were cultured in the presence of TNF-a and anti-Fas antibody. HHV-6 induces apoptosis in CD4^+T cells by indirect mechanisms, as reported recently in HIV-1 infection.
2. We examined the susceptibility to HHV-7 infection of various CD4-negative cell lines into which the cDNA for CD4 was transferred using an adenovirus vector. Out of 13 cell lines transduced with Adex1CACD4, 4 cell lines showed high susceptibility to HHV-7 infection. These data suggest strongly that CD4 is a major component of the binding receptor for … More HHV-7.
3. Two novel Ph chromosome-positive myeloid cell lines, SAS4l3 and SAS527, which possess different hematologic characteristics and show distinct susceptibility to infection of HHV-6, have been established. TPA-treatment of SAS527 which was latently infected with HHV-6B resulted in reactivation of HHV-6. These novel cell lines should be useful for studying the mechanisms of HHV-6- induced hematopoietic failure and HHV-6 latency and reactivation.
4. Although CXCR4 and CCR5 appeared not to be the coreceptors for these viruses, marked down-regulation of CXCR4, but not CCR5, was detected in HHV-6 and HHV-7-infected cells. Down-regulation of CXCR4 resulted in impairment of chemotaxis and a decreased level of elevation of the intracellular Ca^<2+> concentration in response to SDF-1. Northern blot analysis of mRNAs extracted from HHV-6- and HHV-7-infected CD4^+T lymphocytes demonstrated a markedly decreased level of CXCR4 gene transcription, but post-transcriptional stability of CXCR4 mRNA was not significantly altered. Less

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yasukawa,M.,et al.: "Human herpesvirus 7 infection of lymphoid and myeloid cell lines transduced with an adenovirus vector ・・・・・" Journal of Virology. 71. 1708-1712 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inoue,Y.,et al.: "Induction of T-cell apoptosis by human herpesvirus 6" Journal of Virology. 71. 3751-3759 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasukawa,M.,et al: "Apoptosis of CD4+ T lymphocytes in human herpesvirus 6 infection" Journal of General Virology. 79. 143-147 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tohyama,M.,et al: "Severe hypersensitivity syndrome to sulfasalazine associated with reactivvation of human herpesvirus 6" Archives of Dermatology. 134. 1113-1117 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasukawa,M.,et al: "Laternt infection and reactivation of human herpes-virus 6 in two novel myeloid cell lines" Blood. 93. 991-999 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasukawa,M.,et al: "Down-regulation of CXCR4 by human herpesvirus 6 (HHV-6) and HHV-7" Journal of Immunology. in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasukawa, M.et al.: "Human herpesvirus 7 infection of lymphoid and myeloid cell lines transduced with an adenovirus vector containing the CD4 gene" Journal of Virology. 71. 1708-1712 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue, Y., et al.: "Induction of T-cell apoptosis by human herpesvirus 6" Journal of Virology. 71. 3751-3759 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasukawa, M., et al.: "Apoptosis of CD4+ T lymphocytes in human herpesvirus 6 infection" Journal of General Virology. 79. 143-147 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tohyama, M., et al.: "Severe hypersensitivity syndrome to sulfasalazine associated with reactivation of human herpesvirus 6" Archives of Dermatology. 134. 1113-1117 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasukawa, M., et al.: "Latent infection and reactivation of human herpesvirus 6 in two novel myeloid cell lines" Blood. 93. 991-999 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasukawa, M., et al.: "Down-regulation of CXCR4 by human herpesvirus 6 (HHV-6) and HHV-7" Journal of Immunology. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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