1998 Fiscal Year Final Research Report Summary
Analysis of antigens for proliferating T cells in patients with systemic lupus erythematosus.
| Project/Area Number |
09670496
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
内科学一般
|
|---|
| Research Institution | St.Marianna University School of Medicine |
|---|
Principal Investigator |
KATO Tomohiro St.Marianna University School of Medicine Institute of Medical Science Associate professor, 難病治療研究センター, 講師 (80233807)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Keywords | systemic lupus erythematosus / Tcell / antigen-specific reaction / auto antigen |
|---|
| Research Abstract |
Many lines of studies suggested that T cells play pivotal roles in the pathogenesis of systemic lupus erythematosus (SLE). We reported oligoclonal T cell proliferation in peripheral blood of patients with the disease previously. To identify the antigen peptides for the accumulated T cell clonotypes, we developed a T cell receptor cloning method from a single cell for the reconstruction of TCR molecules. Further, we successfully expressed HLA class I molecules with beta-2-microglobulin on the filamentous phages as a single chain for constructing an antigen peptide library. Screening of the library with the reconstructed TCR would determine candidate antigens for the proliferating T cells in SLE.
|
