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1998 Fiscal Year Final Research Report Summary

Appendix and ulcerative colitis

Research Project

Project/Area Number 09670543
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

OKAZAKI Kazuichi  Kyoto University, Associate Professor, Department of Gastroenterology & Endoscoptic Medicine, 医学研究科, 助教授 (70145126)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Takefumi  Kyoto University, Associate Professor, Department of Gastroenterology & Endoscoptic Medicine, 医学研究科, 助手 (20283608)
Project Period (FY) 1997 – 1998
KeywordsIBD / Appendix / MALT
Research Abstract

The mouse has the lymphoid organs like Peyer's patches in the cecum, colon and rectum. However, it is unknown whether they have their own independent or specific functions. Injection of anti-IL-7 receptor antibody into pregnant mice can induce Peyer's patch deficient mice (PPD-mice), and we studied the immunological findings of these mice.
Anti-IL-7 receptor antibody was injected to normal BALB/c mice on the 14th day of pregnancy. Whole-mount immunohistochemical staining showed that the neonatal mice have no Peter's patch but have colonic and rectal patches. They are orally or rectally immunized once a week with 2mg of KLH, or KLH + 10μg of cholera toxin 3 times. Normal BALB/c mice immunized with the same dose were served as control. In some mice, appendectomy was performed to remove the cecal patch: Serum levels of immunogloblins (Igs) and secretary IgA in the small intestinal fluids were measured by ELISA system. Intra-epithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) were analyzed by flow cytometry.
Levels of serum Igs and secretory IgA specific for KLH in the rectally immunized PPD-mice were higher than those in the orally immunized PPD-mice and control mice. The PPD mice without appendix have higher levels of the serum Igs specific for KLH than PPD-mice or control mice. In orally immunized PPD mice, appandectomy induced significant increase in the ratio of serum IgG1/IgG2a specific for KLH. Flow cytometry revealed that B1cells (CD5+,B220 low,s-IgMhigh) are not identified in LPL.
Rectal immunization induce potent immune responses compared with the oral immunization in the mice. Colonic and Rectal patches seem to be independent from intestinal Peyer's patches in the mucosal immune system. Moreover, the appendix may modulate systemic immune response.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Okazaki K,et al.: "Multiple pancreatic masses associated with autoimmunity"Am J Gastroenterol. 93. 99-102 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okazaki K,et al.: "Gastric cancer occurring at the anastomosis after gastrojejunoscopy without gastrectomy"Dig Dis Sci. 43. 898-900 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okazaki K,et al.: "Down regulation of gastric mucin gene expression and its biosynthesis by dexamethasone in the human"J Clin Gastroentyerol. 27. 92-97 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okazaki K,et al.: "Anti-lactoferrin antibodies in autoimmune liver diseases"Am J Gastroenterol. 93. 1334-9 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okazaki K,et al.: "Clinical subgroup of autoimmune hepatitis type 1 sustaining remission without additional drug"Hepato-Gastoroenterol. 45. 1667-72 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okazaki K,et: "Antibodies to Mycobacterium paratuberculosis in Japanese patients inflammatory bowel disease after absorption of serum with M.phrei* with"Dig Dis Sci 1999. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okazaki K,et al.: "Multiple pancreatic masses associated with autoimmunity"Am J Gastroenterol. 93. 99-102 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okazaki K,et al.: "Gastric cancer occurring at the anastomosis after gastrojejunoscopy without gastrectomy"Dig Dis Sci. 43(4). 898-900 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okazaki K,et al.: "Down regulation of gastric mucin gene expression and its biosynthesis by dexamethasone in the human"J Clin Gastroentyerol. 27. 92-97 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okazaki K,et al.: "Vector manometry of the sphincter of Oddi. Efitorials -A new parameter of the pancreato-biliary physiology-"J Gastroenterol. 33(6). 923-924 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okazaki K,et al.: "Anti-lactoferrin antibodies in autoimmune liver diseases"Am J Gastroenterol. 93. 1334-9 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okazaki K,et al.: "Open chest darinage for the postoperative empyema in Boerhaave's syndrome"Dig Dis Sci. 43. 1518-1520 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okazaki K,et al.: "Efficacy of total colonoscopy with a transparent cap in comparison with colonoscopy without the cap"Endoscopy. 30. 444-447 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okazaki K,et al.: "Clinical subgroup of autoimmune hepatitis type 1 sustaining remission without additional drug"Hepato-Gastoroenterol. 45. 1667-72 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okazaki K,et al.: "Antibodies to Mycobacterium paratuberculosis in Japanese patients with inflammatory bowel disease after absorption of serum with M.phrei"Dig Dis Sci. in press. (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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