1997 Fiscal Year Annual Research Report

Reg蛋白を介したガストリンによる胃上皮細胞、胃癌細胞の増殖促進機構の解明

Research Project

Project/Area Number	09670548
Research Category	Grant-in-Aid for Scientific Research (C)
Research Institution	Shimane Medical University
Principal Investigator	木下芳一島根医科大学, 医学部, 教授 (30243306)
Co-Investigator(Kenkyū-buntansha)	千葉勉京都大学, 大学院・医学研究科, 教授 (30188487)
Keywords	ガストリン / 胃粘膜 / 増殖因子 / ECL細胞
Research Abstract	ガストリンが胃粘膜ECL細胞のCCK-B受容体を介してECL細胞からのReg蛋白の発現を促進しているかを明らかとするためにラットを用いた研究をおこなった。ラットにオスモティックミニポンプを用いてガストリンの持続慢性投与をおこなうと胃のECL裁縫からのRegmRNA、Reg蛋白の産生が促進され、この効果は、CCK-B受容体拮抗薬の同時投与で消失した。したがって、ガストリンはECL細胞に直接作用して増殖因子であるReg蛋白の産生を促進することが明らかとなった。また、この時に胃粘膜組織の形態学的な検討をおこなうと、Reg蛋白の産生が促進されている場合にのみ胃粘膜の増殖細胞帯での増殖細胞が増加し、胃底腺組織の過形成がみとめられた。さらに、ヒト生検胃粘膜を用いた検討でも、高ガストリン血症を有する患者ではECL細胞でのRegの産生が亢進しており、血清ガストリン値とReg蛋白産生の間に正の相関があることが明らかとなった。また、高ガストリン血症と高Reg発現を示す例の胃体部粘膜は過形成を示していた。

Research Products
(17 results)

All Other

All Publications (17 results)

[Publications] Kinoshita Y.et al: "Production and activation of hepatocyte growth factor" Digestion. 58. 225-231 (1997)
[Publications] Ding M, et al: "Distribution of prostaglandin E receptors in the rat" Prostaglandins. 53. 199-216 (1997)
[Publications] Kawanami C, et al: "Regenerating gene expression in normal gastric mucosa" J.Gastroenterol. 32. 12-18 (1997)
[Publications] Morimoto K, et al: "Cellular localization of mRNAs for the prostaglandin E" Am.J.Physiol. 272. G681-G687 (1997)
[Publications] Wang HY, et al: "Developmental gene expression of gastrin receptor" Regul Pep. 70. 183-189 (1997)
[Publications] Maekawa T, et al: "Helicobacter pylori induced proinflammatory cytokines" J Lob Clin Med. 130. 442-449 (1997)
[Publications] Kinoshita Y, et al: "Helicobacter pylori-independent chronological change of gastric acid" Gut. 41. 452-458 (1997)
[Publications] Waki S, et al: "Sever muscle damage induced by higy carbohydrate" J.Gastroenterol. 33. 121-124 (1998)
[Publications] Waki S, et al: "Intragastric distribution of NSAID-related ulcers" J.Clin.Gastroenterology. 25. 592-594 (1997)
[Publications] Kishi K, et al: "Pepsinogen C gene expression in gastritis" Biochem Biophys Res Commun. 238. 17-20 (1997)
[Publications] Waki S, et al: "Effect of aging on gastrin receptor gene expression in rat stomach" Peptides. 19. 225-229 (1998)
[Publications] Yoshida M, et al: "Trichuris Trichiura infection" Am J Gastroenterol. 92. 719-720 (1997)
[Publications] Hassan S, et al: "Expression of the proto-oncogene c-kit and its ligand" Dig.Dis.Sci. (in press).
[Publications] Kinoshita Y, et al: "Increased hepatocyte growth factor content in rat stomach" Digestion. (in press).
[Publications] Okada A, et al: "Rat gastric mucosal cells express ICAM-1and proinflammatory cytokines" J Lob Clin Med. (in press).
[Publications] Hassan S, et al: "GGA,antiulcer drug,stimulates hexosamine production in a rat" J Gastroenterol Hepatol. (in press).
[Publications] Maekawa T, et al: "Relation between severity and symptons of reflux esophagitis in elderly" J Gastroenterol Hepatol. (in press).

1997 Fiscal Year Annual Research Report

Reg蛋白を介したガストリンによる胃上皮細胞、胃癌細胞の増殖促進機構の解明

Principal Investigator

木下 芳一 島根医科大学, 医学部, 教授 (30243306)

Research Products

[Publications] Kinoshita Y.et al: "Production and activation of hepatocyte growth factor" Digestion. 58. 225-231 (1997)

[Publications] Ding M, et al: "Distribution of prostaglandin E receptors in the rat" Prostaglandins. 53. 199-216 (1997)

[Publications] Kawanami C, et al: "Regenerating gene expression in normal gastric mucosa" J.Gastroenterol. 32. 12-18 (1997)

[Publications] Morimoto K, et al: "Cellular localization of mRNAs for the prostaglandin E" Am.J.Physiol. 272. G681-G687 (1997)

[Publications] Wang HY, et al: "Developmental gene expression of gastrin receptor" Regul Pep. 70. 183-189 (1997)

[Publications] Maekawa T, et al: "Helicobacter pylori induced proinflammatory cytokines" J Lob Clin Med. 130. 442-449 (1997)

[Publications] Kinoshita Y, et al: "Helicobacter pylori-independent chronological change of gastric acid" Gut. 41. 452-458 (1997)

[Publications] Waki S, et al: "Sever muscle damage induced by higy carbohydrate" J.Gastroenterol. 33. 121-124 (1998)

[Publications] Waki S, et al: "Intragastric distribution of NSAID-related ulcers" J.Clin.Gastroenterology. 25. 592-594 (1997)

[Publications] Kishi K, et al: "Pepsinogen C gene expression in gastritis" Biochem Biophys Res Commun. 238. 17-20 (1997)

[Publications] Waki S, et al: "Effect of aging on gastrin receptor gene expression in rat stomach" Peptides. 19. 225-229 (1998)

[Publications] Yoshida M, et al: "Trichuris Trichiura infection" Am J Gastroenterol. 92. 719-720 (1997)

[Publications] Hassan S, et al: "Expression of the proto-oncogene c-kit and its ligand" Dig.Dis.Sci. (in press).

[Publications] Kinoshita Y, et al: "Increased hepatocyte growth factor content in rat stomach" Digestion. (in press).

[Publications] Okada A, et al: "Rat gastric mucosal cells express ICAM-1and proinflammatory cytokines" J Lob Clin Med. (in press).

[Publications] Hassan S, et al: "GGA,antiulcer drug,stimulates hexosamine production in a rat" J Gastroenterol Hepatol. (in press).

[Publications] Maekawa T, et al: "Relation between severity and symptons of reflux esophagitis in elderly" J Gastroenterol Hepatol. (in press).

木下芳一島根医科大学, 医学部, 教授 (30243306)