1998 Fiscal Year Final Research Report Summary
IDENTIFICATION OF NUCLEAR PROTEIN EXPRESSED IN TRANSACTIVATED ITO CELLS
| Project/Area Number |
09670554
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | KOCHI MEDICAL SCHOOL |
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Principal Investigator |
SAIBARA Toshiji Kochi Medical School, First Department of Internal Medicine, Assistant Professor, 医学部附属病院, 講師 (60145125)
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| Co-Investigator(Kenkyū-buntansha) |
UETA Hiroshi Kochi Medical School, First Department of Internal Medicine, Research Associate, 医学部, 助手 (30284433)
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| Project Period (FY) |
1997 – 1998
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| Keywords | ITO CELLS / LIVER / RAT / STELLATE CELLS / MYOFIBROBLAST / NITRIC OXIDE / CATIONIC AMINO ACID TRANSPORTER |
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| Research Abstract |
Development of liver fibrosis results in hepatic failure, hepatocellular carcinoma, esophageal varices, and spontaneous peritonitis, which are life-threatening complications in liver cirrhosis. Induction of apoptosis in transformed Ito cells maybe a promising way of treatment of chronic liver disease. A new insight into the changes of nuclear protein expressed in the process of transformation may provide an important information for the treatment of liver fibrosis.
Progress in purification of Ito cells enabled us to obtain highly-purified cells and we investigate the process of transformation precisely. In this study, we developed monoclonal antibody for purifying nuclear protein transiently expressed in transformed Ito cells during the process of transformation and the analysis of amino acid sequence revealed it as emelin commonly expressed in myocytes. We need further investigation to recognize the role of transient expression of emelin in Ito cells during transformation.
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