1998 Fiscal Year Final Research Report Summary
A study on the possibility of gastric cancer caused by Helicobacter pylori using human normal epithelial cells in vitro.
| Project/Area Number |
09670567
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Jichi Medical School |
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Principal Investigator |
SATOH Kiichi Jichi Med.Sch., Medicine, Assistant, 医学部, 助手 (50275707)
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| Co-Investigator(Kenkyū-buntansha) |
SAIFUKU Kouji Jichi Med.Sch., Medicine, Lecturer, 医学部, 講師 (60124971)
KIHARA Ken Jichi Med.Sch., Medicine, Assistant, 医学部, 助手 (70195345)
KOJIMA Toshichika Jichi Med.Sch., Medicine, Assiatant, 医学部, 助手
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| Project Period (FY) |
1997 – 1998
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| Keywords | H.pylori / Gastric cancer / in vitro / Apoptosis |
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| Research Abstract |
A working Group of the World Health Organization International Agency for research or cancer conducted in1994 that H.pylori is a group 1 carcinogen in humans. Epidemiological studies have indicated H.pylori infection is associated with gastric cancer. But until now there is few direct proof in experiments. We examined the association between gastric cancer and H.pylori in vitro. We established epitherial cell line derived from human normal gastric mucosa. Using these cells, gastric cancer cell line(KATO-3) and other cell lines, we examined whether H.pylori induce the change of chromosome and apoptosis and the association among cancer, H.pylori and teromerase. H.pylori culture supernatant induced the change of chromosome number in Mm-2T cells and morphological changes in Mm-2T and other cell lines .NH_3 and NH_2CL induced apoptosis and among some H.pylori culture supematant induced apoptosis. Teromerase activity was 20% in intestinalmetaplasia. These data indicate the association between gastric cancer and H.pylori.
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