1998 Fiscal Year Final Research Report Summary

Basic study on DNA vaccine in Hepatitis type C

Research Project

Project/Area Number	09670568
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Gastroenterology
Research Institution	Jichi Medical School
Principal Investigator	MORIYAMA Takashi Jichi Medical School, Department of Medicine, Assistant Professor, 医学部・分子免疫学研究室, 講師 (10240706)
Co-Investigator(Kenkyū-buntansha)	KANEKO Takashi Jichi Medical School, Department of Medicine, Assistant, 医学部・分子免疫学研究室, 助手 (10254913)
Project Period (FY)	1997 – 1998
Keywords	hepatitis C virus / DNA vaccine / cytotoxic T lymphocyte / adjuvant
Research Abstract	We tried to induce a T cell liesponse to hepatitic Cvirus (HCV) core protein by using a DNA vaccine in mice. We constructed plasmids encoding HCV core region under the control of various promotors such as pMAMneo, pEFBOS, and pcDNA3. These plasmids alone induced a helper T cell response only after in vitro restimulation twice. A cytotoxic Tlymphocyte (CTL) response was observed but the magnitude was not strong. Next we immunized mice with a plasmid encoding HCV core region together with an adjuvant plasmid encoding IL-2, GM-CSF, or (CD86. None of these combination resulted in a betterT cell response yet.

(6 results)