1998 Fiscal Year Final Research Report Summary
Basic study on DNA vaccine in Hepatitis type C
| Project/Area Number |
09670568
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Jichi Medical School |
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Principal Investigator |
MORIYAMA Takashi Jichi Medical School, Department of Medicine, Assistant Professor, 医学部・分子免疫学研究室, 講師 (10240706)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Takashi Jichi Medical School, Department of Medicine, Assistant, 医学部・分子免疫学研究室, 助手 (10254913)
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| Project Period (FY) |
1997 – 1998
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| Keywords | hepatitis C virus / DNA vaccine / cytotoxic T lymphocyte / adjuvant |
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| Research Abstract |
We tried to induce a T cell liesponse to hepatitic Cvirus (HCV) core protein by using a DNA vaccine in mice. We constructed plasmids encoding HCV core region under the control of various promotors such as pMAMneo, pEFBOS, and pcDNA3. These plasmids alone induced a helper T cell response only after in vitro restimulation twice. A cytotoxic Tlymphocyte (CTL) response was observed but the magnitude was not strong. Next we immunized mice with a plasmid encoding HCV core region together with an adjuvant plasmid encoding IL-2, GM-CSF, or (CD86. None of these combination resulted in a betterT cell response yet.
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